Effect Of Exercise On Th9?Th17 Positive Cells And Inflammatory Cytokines In Peripheral Blood Of Depressive Patients With Compulsory Depressant Treatment In Baimud Lake,Hunan Province

1 ObjectiveThe effects of exercise on the expression ratio of TH9 and TH17 positive cells and their main inflammatory factors,IL-9 and IL-17A were studied?2 Materials and methodsAccording to the SDS score of self-rating depression scale,60 drug addicts who were in the stage of strong rehabilitation were divided into two groups:the exercise group with depressive symptoms(n?20)and the control group(n=20)with depressive symptoms?No depressive symptoms control group of 20 people,exercise group for 12 weeks of exercise intervention,the control group did not do any intervention,before the intervention and 12 weeks after the end of exercise intervention the next morning to collect all blood samples?Peripheral blood mononuclear cells were isolated by Ficoll and IL-9 IL-17A expression and IL-9 in peripheral blood mononuclear cells were detected by flow cytometryoPercentage of IL-17A double positive cells;The concentration of IL-9 and IL-17A cytokines in plasma was determined by ELISA method?3 ResultsAfter 12 weeks of exercise intervention,the SDS depression score of the depressive exercise group decreased significantly(P<0.01),while the depression scores of the depressive control group and non-depression control group had no significant changes(P>0.05)?The percentage of IL-9 positive expression in peripheral blood mononuclear cells in the exercise group with depressive symptoms after intervention was significantly decreased(P<0.05),the percentage of IL-17A positive expression was significantly increased(P<0.01),and there was depressive symptoms in the exercise groupo Depressive symptoms in the control group plasma IL-9,IL-17A cytokine concentrations were significantly increased(P<0.05)?There was no linear correlation between the SDS depression score and the expression of IL-9 positive cells and IL-9 cytokines,but the%expression with IL-17A positive cells(r?0.63 5,P=0.008),IL-17A cytokine concentration(r?0.534,P?0.011)Positive correlationoThe time of personal drug consumption was positively correlated with the percentage of IL-17A expression in peripheral blood mononuclear cells(r=0.419,P=0.001),and positively correlated with plasma IL-17A cytokine concentration(r=0.452,P?0.002).There is no linear correlation of IL-9 levels?4 Conclusion4.1 Twelve weeks of exercise intervention can effectively improve the depression level of people with strong or depressed depression?4.2 Exercise intervention can significantly reduce the expression rate of IL-9 positive cells in patients with strong obstructive depression,reduce the body's inflammatory response,and can make the IL-17A positive cell expression ratio,IL-9 and IL-17A cytokine concentration benign changes,based on exercise in The effectiveness of immune regulation and functional improvement provides a scientific theoretical basis for rehabilitation training for drug addicts?4.3 The time of drug consumption and degree of depression were positively correlated with IL-17A cytokine levels,and had no correlation with IL-9 cytokine levels.IL-17A may be an important cytokine for inducing new body-specific inflammatory responses?...