| Objective: A rabbit radius fracture model was established to study the effect of Huoxue Jie Gu decoction combined with ikoxin on Serum Osteocalcin(BGP)and fracture site TGF-?1 during the healing process of radial fracture in rabbits.Methods: 60 rabbits were randomly divided into celecoxib group,blank control group and Huoxuejiegu antagonize the celecoxib group,Among them,the group of celecoxib was given Etoricoxib Tablets 200 mg/kg for gavage,2 times,and the blank control group was treated with equal dose of distilled water for 2 times/day.Huoxuejiegu Decoction against celecoxib group first to Etoricoxib Tablets 200 mg/kg to mix by gavage,1 hours after treated with Huoxue soup orally liquid,calculated according to the equivalent calculation formula(body weight is calculated according to 60 kg),the dosage was 2 times of equivalent dose of rabbits,every day 2 times irrigation the stomach.The content of first,second,third,4,respectively in 6 weeks in each group were randomly selected 4 rabbits by enzyme-linked immunosorbent assay in the serum osteocalcin were positive expression of rabbit bone fracture,bone marrow cavity and outer membrane of endometrial TGF-?1 by immunohistochemistry,positive cell average optical density Mean optical density(MOD)value analysis.Results:1.the expression of BGP in serum: Huoxue Jie Gu Decoction antagonized the ikoxib group compared with the blank group.At the 1-6 week,Huoxue Jie Gu Decoction antagonized the level of BGP in the ikoxib group,and the difference was statistically significant(P < 0.05).The comparison between the celecoxib group and the blank group showed that the serum BGP level of the celecoxib was significantly higher at 1-6 weeks,and the difference was statistically significant(P < 0.05).Compared with the ikoxib group,the huoxuelugu Decoction antagonized the ikoxib group and the ikoxib group.At the 1-6 week,the serum BGP level of the Huoxue Jie Gu Decoction antagonized the ikoxib group was significantly higher than that of the ikoxib group(P < 0.05).2.MOD value of TGF-? 1 expression at the end of fracture The membrane of bone TGF-?1 expression values of MOD expression showed that the huoxuejiegu Decoction on display in the comparison between antagonistic test group and the blank group: sixth weeks two group TGF-?1 expression of MOD had no significant difference(P > 0.05),no statistical significance;the 1-4 week huoxuejiegu Decoction against etoricoxib group TGF-?1 expression of MOD was significantly higher than the control group(P < 0.05),the difference was statistically significant.Etoricoxib group compared with the control group showed: first weeks and sixth weeks of TGF-?1 expression of MOD had no significant difference(P > 0.05),no statistical significance;the 2-4 weeks when the huoxuejiegu Decoction antagonist etoricoxib group TGF-?1 expression of MOD was significantly lower than that of the control group(P < 0.05)the difference was statistically significant.Huoxue Jiegu Decoction antagonist etoricoxib group and the comparison between the test group was relying on display: at sixth weeks,two groups of TGF-?1 expression of MOD had no significant difference(P > 0.05),no statistical significance;the 1-4 weeks when the huoxuejiegu Decoction antagonist etoricoxib group TGF-?1 expression of MOD was higher than that of the blank group(P < 0.05),the difference was statistically significant.Endosteal TGF-?1 expression values of MOD showed that the huoxuejiegu Decoction on display in comparison between antagonistic test group and control group: 6W two group TGF-?1 expression of MOD had no significant difference(P > 0.05),no statistical significance;the 1-4 week huoxuejiegu Decoction against etoricoxib group TGF-?1 expression of MOD was significantly higher than the control group(P < 0.05),the difference was statistically significant.Etoricoxib group compared with the control group showed that first weeks of TGF-?1 expression of MOD had no significant difference(P > 0.05),no statistical significance;the 2-6 weeks when the huoxuejiegu Decoction antagonist etoricoxib group TGF-?1 expression of MOD was significantly lower than that of the control group(P < 0.05),the difference is statistical significance.Huoxuejiegu Decoction against etoricoxib group and relying on the comparison between the test groups: display in the 1W two group TGF-?1 expression of MOD had no significant difference(P > 0.05),no statistical significance;the 1-6 weeks when the huoxuejiegu Decoction antagonist etoricoxib group TGF-?1 expression of MOD was higher than that of the blank group(P < 0.05),the difference was statistically significant.The MOD value of TGF-?1 expressed in the bone marrow cavity showed that Huoxue Jie Gu Decoction antagonized the antagonistic effect between the ikoxib group and the blank group.At 1-6 weeks,the MOD value of Huoxue Jie Gu Decoction antagonized the TGF-?1 expression in the ikoxib group was significantly higher than that in the blank group(P < 0.05),the difference was statistically significant.According to the comparison between the two groups,the MOD value of TGF-?1 was not significantly different at first weeks or 6 weeks(P > 0.05),and there was no statistical significance.At 2-4 weeks,the MOD value of TGF-?1 in the ikoxib group was significantly lower than that in the blank group(P < 0.05),the difference was statistically significant.Compared with the ikoxib group,Huoxue Jie Gu Decoction antagonized the ikoxib group and the ikoxib group.At the 1-6 week,the MOD value of Huoxue Jie Gu Decoction antagonized the TGF-?1 expression in the ikoxib group was significantly higher than that in the blank group(P < 0.05),the difference was statistically significant.Conclusion(s): 1.Huo Xue Jie Gu soup and celecoxib can promote the increase of serum BGP content in the process of fracture healing;2.Celecoxib can inhibit the expression of TGF-?1 at the fracture end during fracture healing 3.Huo Xue Jie Gu soup can inhibit the inhibition of the expression of TGF-?1 at the end of the fracture,and increase the expression of TGF-?1 in the fracture healing process. |
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