| Objective: To study the effect of Qingnao Yiyuan Decoction on expression of STAT3 and JAK2 after cerebral ischemia in rats,and to explore the possible mechanism of action target of the signal pathway in the control of cerebral ischemia injury by Qingnao Yiyuan Decoction.Methods: 136 healthy male SD rats,weighing 250 ± 50 g,were randomly divided into 4 groups: the normal control group(31 animals),sham operation group(35 animals),model group(35 animals),and Qingnao Yiyuan group(35animals).Each group was divided into 5 sub-groups according to the five time points of 1d,3d,7d,14 d,and 28 d after ischemic injury.There were 6 rats in each of the 4 sub-groups: 1d,3d,7d,and 14 d.In the 28 d sub-group,7 rats in the blank group,The fake surgery group,the model group and the Qingnaoyi group each had 11 rats.The following processing was made: the rats in the normal control group were feed and drinking normally,also given same volume of distilled water;the rats in sham operation group were simply operated without plug wire animals,also feed and drinking normally after operation and given same volume of distilled water;the rats in model group were narcotized and operated with MCAO after same volume of distilled water was given seven days,the rat model of acute cerebral ischemia injury in rats were made after operation,the rats were irrigated stomach by physiological saline after being awake until the rats were euthanized;the rats in Qingnao Yiyuan group were operated the same methods with model group after irrigated stomach by same volume of Qingnao Yiyuan seven days,the rats were irrigated stomach by Qingnao Yiyuan after being awake until the rats were euthanized.Scores of degree of defect in neurological function in rats were made according to the Longa 5 likert scale.Specimens of each group were collected at different times.The volume of cerebral infarction in rats was determined by TTC staining.The apoptosis of nerve cells was detected by TUNEL.The dynamic changes of JAK2 and STAT3 immunopositive cell expression in ischemic hippocampus were detected by immunohistochemical method.The data were treated by SPSS 24.0 statistical software package.Results:(1)Neural function defect score:At the same time,the symptoms of neurofunctional defect in Qingnao Yiyuan group were improved in different degree compared with those in model group,differences are statistically significant(P<0.05).(2)The results of TTC staining were as follows: White infarct was not found in the normal control group and the sham operation group.Compared with the model group,the volume of cerebral infarction decreased significantly in Qingnao Yiyuan group(P<0.05).(3)TUNEL assay:At the same time,compared with the sham operation group,the number of apoptosis cells on ischemic side of rats in model group and Qingnao Yiyuan group increased significantly,differences are statistically significant(P <0.05).Compared with the model group,the number of apoptotic cells in the Qingnao Yiyuan group decreased significantly,differences are statistically significant(P<0.05).(4)The results of immunohistochemical assay showed that in the same time,the expression of JAK2 and STAT3 immunoreactive cells in normal control group and sham operation group were not statistically significant(P>0.05).The expression of JAK2 and STAT3 immunoreactive cells in the model group and the Qingnao Yiyuan group decreased slowly after the peak of 7d in the ischemic injury.At the same time,compared with the model group,the expression of JAK2 and STAT3 immunoreactive cells in the Qingnao Yiyuan group was significantly decreased,and the difference was statistically significant(P < 0.05).Conclusion: Qingnao Yiyuan Decoction can reduce the expression of JAK2 and STAT3 after cerebral ischemia injury and inhibit the activation of JAK2 and STAT3 signal transduction pathway,which may be one of the mechanisms for its protective effect. |
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