Effect Of Feeding Whole-plant Corn Silage Inoculated With Lactobacilllus Plantrum Or Lactobacillus Buchneri On Growth Performance And Rumen Microflora Of Lambs

In recent years,with the continuous development of the lambs industry,people’s research on lambs is more concentrated.These studies mainly take the effects of different diets of lambs on the growth performance and health of lambs as the main research content and research purposes.Whole-plant corn silage is fed to the lamb as a diet,which can improve the lamb’s feed intake and production performance.Adding lactic acid bacteria additives to corn silage can adjust the microbialflora and speed up the silage fermentation process to preserve the nutrition of silages.Lambs can produce a large amount of microbial protein by feeding such a diet and then through rumen fermentation,which is conducive to the growth and reproduction of rumen microorganisms,thereby improving the production performance of lambs.In this experiment,two strains of lactic acid bacteria（Lactobacillus plantrum LP and Lactobacillus buchneri LB）were added to the whole plant corn silage,and stored for180 days before feeding to lambs.Twenty-four 4-5 month lambs with good physical condition（20-30 kg）were selected and randomly divided into 4 groups of 6 sheep in each group,which were fed in a single pen.The adaptation period（10 d）gradually replaced the test feed,the pre-test period（15 d）,and the formal test（5 d）.After the formal test,all test sheep were slaughtered.By measuring the lamb’s weight,serum biochemical products and other related indicators,rumen fecal flora distribution before and after silage feeding,comprehensive evaluation of the increase in weaned lambs fed with silage inoculated with two lactic acid bacteria weight and health effects.Reveal the interaction between silage inoculated lactic acid bacteria and sheep’s inherent flora and its effect on rumen fermentation mode,and explore the effect of silage inoculated lactic acid bacteria on the growth performance of lambs.The results are as follows:1.After 180 days of silage,the silage quality of each group improved.The dry matter content of the LP group was significantly higher than the other two groups（P<0.05）.The pH of each group was lower than 4,which were the control group（3.89）,LP Group（3.93）and LB group（3.86）.The content of acetic acid in LB group（2.28%DM）and aerobic stability LB group（128 h）were higher than those in other groups.The lactic acid content（5.55%DM）in the control group was higher than the other two groups.2.From the perspective of the changes in the weight of lambs in different diet groups,during the entire test,the daily average weight gain of the lambs in each diet group was silage control group（63.71 g）,straw control group（81.13 g）,LP group（92.47 g）,and LB group（70.7 g）.From the perspective of the feed intake of lambs in different diet groups,the average daily feed intake of lambs in each diet group was silage control group（1.81 kg）,straw control group（1.18 kg）,LP group（1.81 kg）,and LB group（1.75 kg）,the total feed intake was silage control group（57.89 kg）,straw control group（37.69 kg）,LP group（57.96 kg）,and LB group（55.96 kg）.The average daily feed intake and total feed intake of lambs in each silage group were significantly higher than those in the straw control group（P<0.05）.3.From the perspective of the serum biochemical indicators of lambs in different diet groups,the total protein concentration of lambs in each diet group was silage control group（68.1 g/L）,straw control group（68.5 g/L）,LP group（67.3 g/L）and LB group（64.1 g/L）.The straw control group was significantly higher than the LB group（P<0.05）.The triglyceride concentrations of lambs in each diet group were silage control group（0.29 mmol/L）,straw control group（0.64 mmol/L）,LP group（0.32 mmol/L）,and LB group（0.35 mmol/L）.The control group was significantly higher than the LP group（P<0.05）.The concentration of alanine aminotransferase in the lambs of each diet group was silage control group（12.6 U/L）,straw control group（15.4 U/L）,LP group（18.8 U/L）,and LB group（12.8 U/L）.The LP group was significantly higher than the silage control group and the LB group（P<0.05）.The acetic acid content of rumen fluid in lambs in each diet group was between 2.19-2.63mg/ml,the propionic acid content of rumen fluid in lambs in each diet group was between 0.62-0.66 mg/ml,and the content of butyric acid in rumen fluid of lambs in each diet group was between 0.34-0.44 mg/ml.The pH of rumen fluid in lambs in each diet group were silage control group（7.26）,straw control group（6.73）,LP group（6.94）,and LB group（6.83）.The silage control group was significantly higher than the straw control group（P<0.05）.The red blood cell count of each group of lambs was between 5.45-5.88 10¹²/L,the white blood cell count was between 6.16-6.9010⁹/L,and the blood glucose concentration was between 0.98-1.28 mmol/L.4.From the distribution of rumen and feces in different diet groups,it can be seen that according to the analysis of Alpha diversity index,the observed_species,shannon index,and chao1 index of rumen fluid microorganisms,rumen mucosal microorganisms,and rumen solid microorganisms in the straw control group were significantly higher than those in the silage control group（P<0.05）.The fecal microorganisms observed_species and shannon index in the straw control group were significantly higher than those in the LP group（P<0.05）.At the phylum level,there are 25 microorganisms related to the rumen fluid flora,rumen mucosa flora,rumen solid flora,fecal flora,and feed flora of lambs,accounting for more than 95%of the total number of sequences.Among them,Firmicutes and Bacteroidetes are the dominant flora of rumen fluid,rumen mucosa,and rumen solids,all accounting for more than 30%of the total flora;Firmicutes and Bacteroidetes are the dominant flora of feces,accounting for more than 18%of the total flora.Among the feed flora,Firmicutes and Proteobacteria are the dominant flora.At the genus level,There are a total of 301 microorganisms related to lamb fecal flora,rumen fluid flora,rumen mucosa flora,rumen solid flora and feed flora.Prevotella and Ruminococcaceae_unclassified related flora are the dominant flora in rumen fluid,rumen mucosa and rumen solids,and Ruminococcaceae_unclassified related flora are the most abundant flora in feces.Among the feed flora,Lactobacillus is the most abundant flora in the three groups of silage,accounting for more than 28%of the total flora.Bacillaceae_unclassified related flora is the most abundant flora in the straw control group,accounting for the total flora 51.86%.