| Aimed at providing a theoretical basis for the further development and application of Gynostemma pentaphyllum polysaccharides,the study explored the effect of GPP on the antioxidant capacity of aging mice induced by D-galactose.In the process of research,biochemical,enzyme-linked immunoassay and histological techniques were used to determine the total antioxidant capacity in mice;the activity of superoxide dismutase,catalase and glutathione peroxidase in brain and liver tissues as well as active oxygen,malondialdehyde and protein carbonyl content;the content of hydroxyproline in the skin;and the tissue structure changes of the brain,liver and skin.The results showed that:1.The level of antioxidant enzyme activity in mice treated with GPP was enhanced.When the GPP concentration was 200 mg/kg/d,the activity of serum T-AOC was significantly higher than the D-gal group(P<0.05),and there was no significant difference from the blank control group.When the GPP concentration was 100 mg/kg/d,SOD activity in mice brain and GSH-Px activity in liver were significantly higher than the D-gal group(P<0.05),and there was no significant difference from Vc group and Blank control group.When the GPP concentration was 50 mg/kg/d,the CAT activity in the liver of mice was significantly higher than the D-gal group(P <0.05),and there was no significant difference from the Vc group and the Blank control group.2.From the results of the level of peroxidation products in mice,different concentrations of GPP treatment group could reduce the content of peroxidation products in mice.When the GPP concentration was 50 mg/kg/d,the ROS and MDA in the brain and liver of the mice were significantly lower than the D-gal group(P<0.05),and the ROS level in the brain was significantly higher than the Blank control group(P<0.05),and significantly lower than the Vc group(P<0.05),and the ROS level in the liver were significantly lower than the Blank control group and the Vc group,and the MDA levels in brain and liver were comparable to the Vc group and the Blank control group.3.Judging from the results of the organ index of mice which treatmented with different concentrations of GPP.When the GPP concentration reached 200mg/kg/d,the body weight of the mice was significantly higher than the D-gal group(P<0.05).There was no obvious difference between the brain and liver indexes of mice.4.From the results of the tissue structure of pyramidal neurons in hippocampal CA3 area of mice brain,the vertebral neuron cells were increased compared with the D-gal group when the concentration of GPP was 50 mg/kg/d and 100 mg/kg/d.Most of the cells were neatly arranged,the layers were tight,the nucleoli were deeply stained,and the overall structure was not significantly different from the Blank control group and the Vc group.5.From the results of the tissue structure of mice liver tissue,the size of hepatocytes and nuclei were slightly reduced compared with the D-gal group.when the GPP concentration was 200 mg/kg/d,the hepatic sinusoids were reduced as well as the hepatic cells were tightly connected.The liver cells with fat particles were significantly reduced.6.From the results of the tissue structure of mice skin,when the concentration of GPP was 50 mg/kg/d,the epidermis of the mice had a uniform thickness,a complete structure,a clear boundary with the dermis layer,and collagen fibers was neat and the gap becomes smaller compared with the D-galgroup and other dose groups of GPP.The above results indicated that Gynostemma pentaphyllum polysaccharide had a good enhancement effect on the activity of antioxidant enzymes in the liver,brain,skin and serum of aging mice induced by D-galactose,and could reduce the body damage caused by peroxidation products,enhance the antioxidant capacity of aging mice. |
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