Screening Of Molecular Markers For Purity Identification Of Radish Germplasm And Hybrids

As one of the origins of radishes,China has abundant germplasm resources.Understanding the genetic relationship of these resources can effectively guide breeding.Molecular marker analysis can be use to quickly and reliably analyze the genetic relationship between germplasm.On this basis,it can also construct a molecular marker fingerprint of germplasm resources to assist in the protection of varieties.In addition,with the improvement of living standards,people have higher requirements for the uniformity of vegetable quality and appearance,and the purity of vegetable seeds has been paid more and more attention.Hybrids produced by traditional breeding methods usually need to be planted for one season to ensure the purity of the seeds before they can be sold,which not only increases the workload of breeding,but also increases the cost of seed storage and management.The use of molecular marker assisted technology for purity identification of hybrids only requires collecting leaves at the seedling stage and extracting DNA for testing,which greatly reduces costs and cycles.However,there are relatively few studies on the use of molecular markers to construct fingerprints and identify the purity of hybrids in radishes.Therefore,in this study,118 markers were used to analyze 110 radish germplasm resources.By screening markers,the phylogenetic relationship of 110 materials was analyzed,and a fingerprint of germplasm was constructed.From the 118 pairs of primers,2 pairs of primer pairs were selected for the purity identification of the hybrid ?sheng luo cui yu?;4 pairs of primers were selected for the hybrid ?sheng luo yan yu? for the purity identification of the hybrid and verified.The research results obtained are as follows:1.Using 110 excellent domestic and foreign radish germplasm as material,56 pairs of primers with high polymorphism and clear band pattern were selected from 118 pairs of primers,and 130 bands were amplified from 110 materials,with an average of 2.3.The ratio of polymorphism is 100%,the range of polymorphic information(PIC)is 0.30-0.55,the primers for moderate polymorphism include 53 pairs,and the primers for high polymorphism are 3 pairs.Using the selected markers for genetic diversity analysis,the genetic distance of 110 materials ranged from 0.54 to 0.99.At the clustering threshold of 0.54,110 radish germplasms were divided into two categories;At the threshold of 0.66,110 materials can be divided into 11 categories,including 2 major categories and 9 minor categories.2.From 56 pairs of primers with high polymorphism and clear band pattern,18 pairs of core primers can distinguish 110 radish germplasms,among which 3 pairs of primers RsSSR108,RsIBP18 and RsSSR109 can separately distinguish 4 germplasms 17 pairs of primer combinations can distinguish 106 germplasms,and fingerprint identification codes of 110 radish germplasms were constructed.3.Through the study of purity identification of hybrids,2 pairs of primers were screened for purity identification of hybrid ?sheng luo cui yu? and 4 pairs of primers screened for hybrid ?sheng luo yan yu?.The results of the study showed that the selected primers could amplify the bands that had a codominant relationship with their parents,and the primer amplification band patterns were consistent with the phenotypes of field hybrid materials.