Inheritance And Molecular Marker Of CMS In Radish

In this study,we have investigated the rules of inheritance of cytoplasmic male sterility(CMS)in radish and observed 3 sets of F2 segregating populationand 3 sets of first backcross generation individuals. We obtained the radish material CMS from the Cabbage Study Group of Horticultural College,Northwest Agricultural and Forestry University. The segregating population in BC1 and in F2 was found to respectively fit a segregation ratio 1:1 and 3:1 for male fertile and sterile types,respectively,indicating that the male fertile gene is a single dominant gene.The extraction of high quality of integrated DNA by means of improving the method of CTAB provided an advantage for the followed reaction of PCR. Further research was carried on with RAPD and SRAP molecular marker. The plants in radish CMS were taken as the material for molecular marker,and mixed equivalently to get the sterile mixture and fertile mixture screening by method of BSA. 500 primers were used to the PCR amplification of the study of polymorphism between the two mixtures. The analysis showed that the primer S1344 and S88 can exhibited stable polymorphism difference. We obtained 2 markers,S88-1526 and S1344-415,closely linked to the fertile gene in radish, the genetic distance of which is 4.2cM and 6.9cM,respectively. Since the genetic distance between S1344-415 and target gene is 6.9 cM,and 9.1 between S88-1526 and S1344-415, we could conclude that S88-1526 and S1344-415 were on the two sides of the target gene. This marker could apply to the marker subsidiary slection of the male fertile restorer gene.The sequences homologies of the S88-1526 and S1344-415 were analyzed using the BLAST program. The result showed that the sequence homology between S88-1526 and the KBrB091J13 clone in cabbage BAC library was75%,and 75% between the S1344-415 and the KBrB011P07 clone in cabbage BAC library,indicating that these two sequences are new sequences in radish.88 pairs SCAR primers were used to amplified the B20×A20. The primer composition of me5-em4 could amplify a 100bp band which was named as me5-em4-100 in 28 of the 34 male fertile plants,and the same as in 9 of the 38 male sterile plants. The genetic distance between the me5-em4-100 and target gene is 20.8cM.