The Genetic Diversity Analysis And Fingerprinting Construction Of The Rice Parental Resources

Rice(Oryza sativa) is one of the most important crops and major food for many people in the world. In China, the yields of rice have increased greatly since the application of hybrid rice and research for super-rice. So it is very important to study the genetic diversities and relations among the parents of the rice which has provided the main materials for rice breeders.The genetic diversity of more than360hybrid rice pro-sources of Hunan WeiMingKaiTuo was analyzed through the use of the microsatellite marker,48pairs of SSR primers was selected, an average of four pairs of12chromosomes in the rice. The detail results were as follows.Fourty eight pairs of SSR primers all shows a polymorphism, detected a total of181polymorphic locus, an average of3.77alleles,3.77alleles in the indica and3.60in japonica, the genetic diversity index of the indica and the japonica are0.7104and0.5887, respectively, according to the average gene diversity and the number of alleles in the single loci of parental resources, the genetic diversity of the indica is higher than the japonica.The cluster analysis, from some angles of different subspecies (indica and japonica) and different geographic sources (provinces), shows that all the tested materials can be well divided into two categories, indica subspecies and Japonica subspecies, the result is consistent with pro-source relations. Combined with recent studies, it furtherly shows that the classification of indica and japonica by using SSR markers has a certain degree of accuracy.In addition to SSR markers, cluster analysis of agronomic traits do not reflect genetic discrepancy between varieties fundamentally. Agro-nomic traits is easier to be influenced by the environment.The cluster analysis of part of the parental resource (CMS, TGMS, maintainer lines and restorer lines) can be seen from the results, CMS and TGMS were basically clustered into one class, respectively,which shows the genetic discrepancy of CMS and TGMS are small. Male sterile lines and restorer lines were clustered into two groups, maintainer lines do not show the trend of centralized, the clustering results are consistent with the genetic diversity index, but The results show that the farther genetic distance between the restore liness and male sterile liness reflects, to a certain extent, positive correlation between genetic distance and heterosis, which is used to guiding rice breedingFingerprinting plays a great role in the ownership of protected species and so on.24pairs of primers for fingerprinting were selected. Without field hybrid experiments,the accuracy of fingerprinting by using the24pairs of primers requires further validation.