| Yellowhorn（Xanthoceras sorbifolium Bunge）is a deciduous shrub or tree of the genus Xanthoceras of the Sapindaceae family and a unique woody oil plant in northern of China.Yellowhorn is also a multi-purpose tree species for flowering ornaments and pharmacological value.At present,Yellowhorn was mainly propagated was by seed or grafting and it limited the extension of the excellent varieties.Under this background,the paper studied on the cutting propagation and tissue culture of yellowhorn.The results are as follows:（1）Cuttage experiment on hard branch of yellowhorn,the highest rooting rate was42.33%in strain’46321’treated with 1000 mg·L-1 NAA for 10 seconds;40.00%in strain’WF12’treated with 500 mg·L-1 NAA for 2 hours;67.67%in strain’WF17’treated with 100mg·L-1 IBA for 2 hours;47.67%in strain’WF18’treated with 300 mg·L-1 NAA for 1 hour.（2）Cutting experiment of young branches of yellowhorn,the highest rooting rate was75.00%in strain’46321’treated with 500 mg·L-1 IBA for 2 hours;81.33%in strain’WF12’treated with 500 mg·L-1 IBA for 2 hours;86.33%in strain’WF18’treated with 500 mg·L-1IBA for 2 hours;79.67%in strain’LY1’treated with 500 mg·L-1 NAA for 2 hours.（3）The soluble sugar content of cuttings showed different trends during rooting.The NAA and IBA groups showed a"slow rise-down-rise"trend,while the ABT and control groups showed a"decline-rise"trend.In the early and late stages of rooting of yellowhorn,the soluble sugar content of the hormone-treated group was significantly higher than that of the control group.The hormone treatment was beneficial to increase the soluble sugar content,especially at the beginning of callus formation and rooting;The content of soluble protein showed a trend of"down-up-down"The soluble protein content of the hormone-treated group was higher than that of the control group,which may be the reason for slow growth and low survival rate of cuttings in the control group.（4）During the rooting process of yellowhorn,POD activity first increased and then decreased;the activity of PPO was always on the rise;IAAO activity showed different trends during rooting of yellowhorn.The treatment group and the ABT treatment group showed a trend of rising first and then falling,and the control group showed a trend of falling first and then rising and then falling.（5）In the tissue culture experiment using stems of yellowhorn,the best time for material extraction is mid-May;the best disinfection method for explants is:disinfection with75%alcohol for 3 seconds,rinse with sterile water 3 times,and Sterilize with 3%NaClO for3 minutes,rinse 3 times with sterile water,and finally disinfect the surface with 0.1%HgCl for 30 seconds,rinse 3 times with sterile water;Adventitious buds were induced in 1/2MS medium supplemented with 1.0 mg·L-1 6-BA and 0.9 mg·L-1 NAA.The induction rate was88.33%,the germination time was 7 days,and the pH was 5.8;Axillary buds were added to1/2 MS medium supplemented with 1.5 mg·L-1 6-BA and 0.6 mg·L-1 IAA.The value-added rate was 3.91.（6）Tissue culture experiment was carried out with the young embryo of yellowhorn as experimental material.Callus induction was performed in 1/2 MS medium supplemented with0.5 mg·L-1 6-BA and 1.0 mg·L-1 2,4-D and 0.4 mg·L-1 NAA.The induction rate was 81.67.The callus was light green and loose;Adventitious bud induction was performed in WPM medium supplemented with 1.5 mg·L-1 6-BA and 0.6 mg·L-1 IBA and 0.7 mg·L-1 NAA with an induction rate of 85.45%;Adventitious buds were added in WPM medium supplemented with 1.5 mg·L-1 6-BA and 0.4 mg·L-1 NAA,and the increase rate was 3.40;Adventitious root induction was performed in MS medium supplemented with 1.0 mg·L-1 IAA and 0.4 mg·L-1NAA.The average number of roots was 4.60.30 g?L-1 sucrose was added to all media,and the pH was adjusted to about 5.8.The average induction rate is 58.31%. |
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