Safety Evaluation Of "Qinbaidu" Granule And Its Effect On Growth Performance Of Hemp Ducks

Cortex fraxini,Chinese pulsatilla and Eucommia ulmoides are traditional Chinese medicines which have antibacterial,anti-inflammatory,anti-oxidation and immune enhancement effects.Previous studies have shown that the effective extract of compound“Qinbaidu”powder has a good therapeutic effect on mice artificially infected with Riemerella anatipestis,and is prepared into granules.In this experiment,the toxicological safety of“Qinbaidu”granule was systematically evaluated and added as feed additive.The effects of“Qinbaidu”granule on growth performance,immune function and antioxidant capacity of hemp ducks were further discussed.The nutritional effects of different doses of“Qinbaidu”granule on ducks were studied from many aspects,which provided a scientific theoretical basis for adding Chinese herbal medicine additives to the formulated diet of ducks.The main contents and results of this study are as follows:1 Safety evaluation of“Qinbaidu”granuleAccording to the compilation of guiding principles for veterinary drug research technology,the safety pharmacology study on rats,acute toxicity test in mice and 30-day subchronic toxicity test in rats were carried out to evaluate the safety of“Qinbaidu”granules.In safety pharmacological tests,the effects of“Qinbaidu”granule on the central nervous system of rats were observed by rod climbing test,and the cardiovascular system and respiratory system were measured by BL-420F multi-channel physiological signal acquisition and processing system.The results showed that the high?16.00 g/kg?,middle?8.00 g/kg?and low?4.00 g/kg?doses of“Qinbaidu”granule had no significant adverse effects on three system of rats after 7 days of intragastric administration.Acute toxicity test showed that when the doses greater than 5000 mg/kg,the mice showed normal performance and no death.LD₅₀was determined to be more than 5000 mg/kg,which proved that the compound“Qinbaidu”granule was a non-toxic substance.Subchronic toxicity test showed that no obvious toxic reaction was observed in the high dose group?16.00 g/kg?,middle dose group?8.00 g/kg?and low dose group?4.00 g/kg?of“Qinbaidu”granule after 30 days of intragastric administration.High concentration of“Qinbaidu”granule can reduce the feeding of rats,while low concentration of“Qinbaidu”granule can increase the feeding.Generally speaking,there is no adverse effect on the growth of rats.After 30 days treatment,there was no significant difference in the hematological parameters between the experimental group and the control group,except for the total protein content of the low dose group“Qinbaidu”granule was slightly different from that of the normal control group,other indexes had no evident differences.Histopathological examination results showed that high dose of“Qinbaidu”granule could cause reversible degeneration in liver,kidney and ovary,and had no damage to other tissues.2 Effect of“Qinbaidu”granule on growth performance of hemp ducksA total of 90 one-day-old hemp ducks were randomly assigned into 5 groups,blank control group fed with basal diet,positive control group added 0.5%Astragalus polysaccharide to the basal diet,low,medium and high dose groups added 0.25%,0.5%and1%“Qinbaidu”granule respectively.At the age of 7 days,the drug was administered by mixing for 22 days,and the samples were collected at 14,21 and 28 days of age to study the effects of“Qinbaidu”granule on growth performance,immune function and antioxidant function of hemp ducks.The results showed that adding different doses of“Qinbaidu'granule to the basic diet had no significant effect on the average body weight,average daily feed intake,average daily gain and feed gain ratio of the hemp ducks.3 Effect of“Qinbaidu”granule on immune function of hemp ducksThe immune organ index of the ducks was calculated,the serum concentrations of IgG,IgM,IgA,IL-2 and IFN-?of the hemp ducks were detected by ELISA.The results showed that the serum concentration of IgG,IgA,IgM and IL-2 could be increased by adding0.25%-1%“Qinbaidu”granule to the diet.At the same time,0.5%“Qinbaidu”granule could significantly increased the relative weight of thymus gland,thus effectively enhance the immune function of ducks.4 Effect of“Qinbaidu”granule on antioxidant function of hemp ducksTo evaluate the antioxidant capacity of“Qinbaidu”granule on hemp ducks,serum total antioxidant capacity?T-AOC?and glutathione peroxidase?GSH-Px?activity were determined by chemical colorimetry,and serum concentration of malondialdehyde?MDA?was determined by thiobarbituric acid?TBA?method.The results showed that 0.25%-1%“Qinbaidu”granule could increase serum GSH-Px activity,and 0.5%addition could reduce the serum concentration of MDA,thus effectively enhance the antioxidant capacity of duck.In conclusion,“Qinbaidu”granule have the high security.Adding“Qinbaidu”granule to the basal diet can significantly enhance the immune function and improve the antioxidant capacity of the ducks,and has a good nutritional effect on the ducks.