| Canine hip dysplasia(CHD)is a common orthopedic disease that is affected by environmental and genetic factors.It can occur in dogs of any breed and different body sizes,with medium and large dogs aged from 2 to 6 months being the most susceptible,and the morbidity rate varies with breeds.At present,researches on CHD mainly focus on X-ray diagnosis,disease degree classification and genetic breeding whereas few studies about the analysis of pathogenic genes have been reported.In this study,59 suspected dogs of CHD were collected from pet hospital in chengdu from July 2017 to October 2018,and 23 cases were diagnosed with CHD by imaging,with the detection rate 38.98%.The Norberg angle,acetabular abduction angle,neck-shaft angle and eccentricity of 59 dogs were measured,combined with the analysis of its basic information(age,weight and gender,etc.)which showed the detection rate of large dogs is highest(47.22%).Weight has an effect on the left acetabulum outreach Angle and eccentricity: the left acetabulum abduction angles of CHD dogs within 15-30 kg is bigger than that of CHD dogs under 15 kg,with extremely significant difference(P < 0.01).For both dogs within 15-30 kg and dogs under 15 kg,the eccentricity of the left side of CHD group were smaller than normal group,with extremely significant difference(P < 0.01),and dogs under 15 kg on the left side of the eccentricity of CHD were significantly smaller than 15 ~ 30 kg dogs and dogs above 30 kg on the left side of the eccentricity of CHD(P < 0.05).Age affects the left acetabular abduction Angle: the left acetabular abduction Angle of CHD dogs aged 12-24 months and above 24 months was significantly bigger than that of the corresponding normal group(P < 0.05),and the left acetabular abduction Angle of CHD dogs aged 24-24 months was significantly smaller than that of CHD dogs aged 12-24 months(P < 0.01).Body shape affects the left cervical Angle and acetabular abduction Angle: the left cervical Angle in the medium-sized CHD dogs was significantly bigger than that in the corresponding normal group(P < 0.05),and the left neck-shaft angle in the medium-sized CHD dogs was also significantly bigger than that in the CHD group of large dogs(P < 0.05).The left acetabular abduction Angle of medium-sized CHD dogs and large dogs was significantly bigger than that in the corresponding normal group(P < 0.05),and there was a significant difference in the left acetabular abduction Angle among small dogs,medium-sized dogs and large dogs(P < 0.05).The contents of SOST,PON2,FGF-23,COL15A1 and FN1 in the plasma of 21 German shepherd dogs(5 normal and 16 CHD)were determined by ELISA.The content of FGF-23 in the CHD group was significantly higher than that in the normal group(P<0.05),and the content of COL15A1 in the CHD group was significantly lower than that in the normal group(P < 0.05).LAMA2(CFA1:70997779),LRR1(CFA8:29247021),SCR(CFA24:28944481),COL6A3(CFA25:51031100)and FN1(CFA37:25095511)SNP loci were detected in 21 German shepherds.There was no significant difference between the normal group and the control group.29247021)SNP site of C/C type.However,in clinical practice,CHD is mainly characterized by A/A type of LAMA2(CFA1:70997779),C/C type of LRR1(CFA8:29247021)and A/A type of FN1(CFA37:25095511).The results of this study suggest that large dogs are susceptible to CHD.The increase of FGF-23 and the decrease of COL15A1 in plasma may be a regulation for German shepherd dogs with CHD,which provides a new idea for the diagnosis of CHD of German shepherd dogs.There are three SNP types in CHD of German shepherd dogs,included A/A type of LAMA2(CFA1:70997779),C/C type of LRR1(CFA8:29247021)and A/A type of FN1(CFA37:25095511),which can potentially be used as a screening basis for the breeding of German shepherd dogs. |
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