Impact Of Several Welfare Intervention Measures On Stress-Related Index In Mice

Objective To study impact of frequently-used material intervention measures towards stress-related index of laboratory animals.Methods Sixty weaning male KM mice were randomly assigned to five groups: A is routine feeding group,B is nutritional intervention group,C is sunflower seed group,D is toy group,E is incense group,and each group has 12 mice.Food and water intake was measured every day.Body weight was measured every two days.Blood sample was collected37 days later from the lateral vein in mouse tail and blood routine examination has been performed.mRNA index of GR,POMC,nNOS,BDNF,NMDA,TH and TPH in brain tissues of the mice was measured by fluorescent real-time PCR method and difference between material intervention groups and general feeding group was compared.Results Weight gain of the other groups reduced compared to group A.As for food intake,group B reduced compared to group A,while group D and E increased.As for water intake,group B reduced compared to group A,while group D and E increased.As for the level of BDNF mRNA expression,comparing with group A,group F and E have increased obviously,while group C has reduced to some extent.As for the level of GR mRNA expression,comparing with group A,group D has reduced obviously,while group C and E have reduced to some extent.As for the level of POMC mRNA expression,comparing with group A,group D has reduced to some extent,while other groups have reduced obviously.As for the level of NMDA mRNA expression,comparing with group A,group D has increased obviously.As for the level of TH mRNAexpression,comparing with group A,group B and C have reduced to some extent.As for the level of TPH mRNA expression,comparing with group A,group C has reduced to some extent.Hematological parameters have no obvious difference among all groups.Conclusions Food and water intake will go up sharply,ability to resist fear and anxiety will be enhanced obviously,learning and memory ability will be reinforced largely and stress-related index will be reduced evidently if toys are placed.Food and water intake will go up,ability to resist fear and anxiety will be enhanced obviously and stress-related index will be reduced evidently if incenses are used.