Molecular Marker-assisted Selection Of Rice Breeding Progeny Materials With Excellent ?-aminobutyric Acid Content

In this study,the hybrid F4 generation of Ningnongheijing/Gaoliangdao-1 was used as the experimental material,and the SSR marker pair was used based on the three QTL sites controlling the y-aminobutyric acid content in the rice grain obtained in the early stage of the laboratory.F4 generation genotype detection,obtained different gene types,and genotype combination classification,based on this,using high performance liquid chromatography to determine the content of y-aminobutyric acid in F4 generation different genotype combination seeds,combined with F2 generation The correlation between different generations and their ?-aminobutyric acid content was analyzed.The correlation between the content and grain traits was analyzed by detecting the grain traits of F4 generation,and the progeny materials rich in ?-aminobutyric acid were screened.The main findings are as follows:1.In the F4 population,the content of ?-aminobutyric acid in the grain was different,the highest content was 12.89 mg/100g,the lowest content was 2.99 mg/100g,and the average content was 6.82 mg/100g,which was between the parents.2.Frequency analysis of ?-aminobutyric acid content of individual grain in F4 population:?-aminobutyric acid content was continuously distributed,and 69 individual plants were concentrated in 5?10 mg/100g,accounting for 109 of total population 63.3%;normal distribution of ?-aminobutyric acid content in F4 generation grain,the test found that:y-aminobutyric acid content of F4 generation single grain basically accorded with normal distribution,and there was obvious super-parent phenomenon.3.In all combinations of F2 and F4 generations,?-amhobutyric acid,which represents a high-content homozygous genotype combination(BBA combination),has the highest mean value,representing a low-level homozygous genotype combination(AAB combination)in ?-The detection results of aminobutyric acid were the lowest,and the results were consistent with the genotype.The significant difference analysis showed that there was a significant correlation between ?-aminobutyric acid content and genotype combination.4.Compared with the F2 generation,the average content of y-aminobutyric acid in the BBA combination of the F4 generation increased,while the average content of ?-aminobutyric acid in the AAB combination of the F4 generation decreased,and the variation range of y-aminobutyric acid content of the individual plants in the combination is reduced.In particular,the conformity of low-content homozygous genotype materials increased from 53%to 75%,and the results showed that low-content homozygous materials tend to be stable.5.The correlation between ?-aminobutyric acid content and grain showed that the content of y-aminobutyric acid was significantly positively correlated with its relative embryo weight,and was significantly negatively correlated with 1000-grain weight,and was significantly negatively correlated with grain thickness.The color showed a significant positive correlation;the results showed that The material with larger relative embryo weight,moderate grain weight and 1000-grain weight,and darker color can be used to provide a basis for screening progeny materials with high y-aminobutyric acid content.6.According to the correlation analysis between y-aminobutyric acid content and grain traits in F4 generation seeds,combined with the analysis results of genotypes,the single plants with number 1,6,7,and 8 combinations of BBA combinations were obtained as comprehensive traits.Excellent offspring material.