| H.diffusa used as immune drugs for thousands of years,has also been included in the medicinal maternal standard.In 2010,H.diffusa has been contained in the"Chinese Pharmacopoeia" as one of the most mpormtant Chinese herbs.This experiment studied the quality identification and discussed the effect of Hedyotis diffusa polysaccharides powder on growth performance and immune function in mice,as well as the toxic effect in rats.1.The quality identification of H.diffusa polysaccharide powderIn order to control the quality of the products,we used the TLC r qualitative identification of H.diffusa polysaccharide powder.And this study used ultraviolet-visible spectrophotometry to further control the content of H.diffusa polysaccharide powder.In addition,the experiment studied the the stability of the products through the acceleration and long-term experiment.The results showed that the product(batch number:110318,110320,110322)from Aether Centre Enterprises Ltd do contain H.diffusa herb ingredients.We chose the 490 nm due to wavelength measuring,and draw the standard curve y=0.0804x+0.0079;The results showed that the equipment was good and precision,the test sample was stability,and this determination method was repeatability and feasible;The recycling experiment results showed that the content determination method was scientific and accurate and the average content of the products was 321.7 mg/g.In the condition of 40±2? and 75±5%,products properties were tan,moisture rose slightly,and reponed positively,the content of polysaccharide declined slightly.In the condition of 25?2? and 60±10%,products properties were tan,moisture rose slightly,and reponed positively,the content of polysaccharide declined slightly.In conclusion,the H.diffusa polysaccharide powder.(batch number:110318,110320,110322)from Aether Centre Enterprises Ltd do contain H.diffusa herb ingredients.The storage condition of H.diffusa polysaccharide powder was seal and moistureproof for 18 months.2.Effects of H.diffusa polysaccharide powder on body weight and immunity in miceSixty mice were randomly divided into four groups with 15 mice each.And the high,medium,low dose group were given 150,100,50 mg/kg body weight of products aqueous solution,the control group were gave distilled water.After 7,14 and 21 days,we collected blood for the determination of the body weight,spleen coefficient,HI antibody titer,lymphocyte stimulus index and the experiment of phagocyte.The study found that the body weight of high-dose and medium-dose group were increased significantly compared to the control group after the first day immunity(P<0.05),and after the days of 7,14,21,the body weight of three dose group were all increased significantly.In the index of HI antibody titer,lymphocyte stimulus index(P<0.05),the high-dose and medium-dose were evaluated significantly compared to the control group after the days of 7,14 days immunity(P<0.05),the three dose group were all increased significantly after 21 days immunity(P<0.05);There was a significance increase in spleen coefficient phagocyte devouring percentage and index of high-dose and medium-dose group compared with the control group(P<0.05).In conclusion,H.diffusa polysaccharide powde could remarkably improve the level of cellular immunity and humoral immunity in mice,also significantly improve the macrophage phagocytosis and spleen index,as well as the body weight of mice.3.The toxicity experiment research of H.diffusa polysaccharide powder.The studies were included acute and long-term toxicity of H.diffusa polysaccharide powder research.In the acute experiment,we first did the preliminary experiment.30 mice were randomly divided into 5 groups with 6 mice each.Five groups were gave 50mg/kg?100mg/kg?500mg/kg?2500mg/kg and 5000mg/kg respectively of the products aqueous solution,each mice were gave the amount of 0.8ml/20g.we observed the mice for 7 days after the treatment and recorded the numbers of dead mice.In the maximum tolerance test,20 mice were randomly divided into 2 groups with 10 mice each.The control group were fed by physiological saline,the experiment group were fed by H.diffusa polysaccharide powder aqueous solution.each mice were gave the amount of 0.8ml/20g twice in one day.We observed for 7 days after the treatment and recorded the numbers of dead mice,food intake and other behavior activities.After cervical vertebra executed in mice,we observed the pathological changes of main organs with naked eye.In the long-term experiment,80 mice were randomly divided into 4 groups with 20 mice each.The expeiment groups were gave 1000mg/kg.50000mg/kg.250mg/kg respectively of the products aqueous solution,the control group were fed by physiological saline.Each group of rats fed by 10 ml/kg body weight for 28 days in a row,we recorded the body weight dead mice,food intake and other behavior activities after 14 days.At the days of 28 days and 42 days 10 rats of each group were executed to determinate the hematology,biochemical indexes and organ-body ratios.The studies found that the mice were health by 5000mg/kg body weight dose in the preliminary experiment.And in the period of one week of observation in the maximum experiment,all the mice were alive by 10000mg/kg body weight dose.All the organs were showed normal by dissection with naked eyes.In the period of 28 days of H.difusa polysaccharide powder treatment,there was no significance change on the appearance,behavior activities,stool properties,and body weight in experiment group compared with the control group(P>0.05),as well as the hematology(P>0.05),biochemical indexes(P>0.05).After 14 days of drug withdrawal,there were no significance change on all the indexes in in experiment group compared with the control group(P>0.05).In conclusion,the maximum toleranc of H.diffusa polysaccharide was 10000 mg/kg body weight in mice,and there was non-toxic in rats,could be use for a long-term. |
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