Study On Cutting And Tissue Culture Of Wild Cherry Blossom Cerasus Duclouxii In Shennongjia

Cherry blossom is a ornamental plant species that belongs to Rosaceae,Prunoideae,Cerasus.Cherry blossom has important ornamental value for gardens.China has abundant wild cherry resources.Shennongjia Forestry District is located in the northwestern border of Hubei Province.It is a transitional area between the western plateau and the low hills of the east of China.It belongs to the north subtropical climatic.Shennongjia Forestry District has the only larger original forest distribution area in the central China,it is an international famous ecological tourism resort,with rich wild garden plant resources.In the early spring,Shennongjia wild cherry blossoms are scattered in the jungle,forming a unique cherry blossom landscape.In order to develop and utilize the excellent germplasm resources of Shennongjia wild cherry blossoms,scientifically introduce cherry blossom varieties,and create the characteristic cherry blossom landscape in Shennongjia forest area.this paper conducts a preliminary investigation on the wild cherry blossom resources of Shennongjia,and selects the wild Cerasus duclouxii plants with high ornamental value for cutting propagation and tissue culture research,at the same time,the Cerasus.serrulata var.lannesiana‘Sekiyama’cultivated on the campus of Yangtze university campus in jingzhou was used as a comparative cutting and tissue culture experiment,In order to find out the technology suitable for the asexual reproduction of wild Cerasus duclouxii,for the wild cherry blossoms in Shennongjia forest area provide technical support for seedling cultivation and preservation of excellent germplasm resources.In addition,this paper also studies the growth adaptability of the Cerasus serrulata and the Cerasus yedoensis‘Yedoensis’that were introduced from Wuhan University in Shennongjia Forestry District,aiming to provide theoretical and practical basis for the introduction of cherry blossoms inShennongjia Forestry District.The main findings are as follows:1.Through literature research and field investigations,Shennongjia Forest District has 13 kinds of wild cherry blossoms,they are:C.tomentosa、C.setulosa、C.conmdinae、C.trichostoma、C.cantabdgiermis、C.szechuanica、C.scopulorum、C.duclouxii、C.tatsienensis、C.subhirtella、C.glabra、C.stipulacea、C.yedoensis.The field investigation found c.subhirtella、c.glabra、c.stipulacea、c.yedoensis、C.scopulorum、C.duclouxii.Shennongjia wild cherry blossoms have a wide range of altitudes,and many sporadic growths occur in sunny valleys,streams,and roadsides.A few patches are distributed in secondary forests such as hillsides and sunny slopes.The habitats are not subject to human destruction or human disturbance.It was found that there is one excellent single plant of C.duclouxii,which is tall and straight,pale pink color,soft and clean,and high greening and ornamental value.2.In March 2017,June 2017 and January 2018,the experiment of cutting seedlings of C.duclouxii cuttings was carried out with river sand as the substrate.The base of C.duclouxii cuttings was treated with 10 different treatments:IBA50 mg/L for 2h,IBA80mg/L for 1.5h,IBA100 mg/L for 1h,NAA50 mg/L for 15 min,NAA80 mg/L for 10 min,NAA100 mg/L for 5 min,rooting powder 200 mg/L soak for 1h,rooting powder 500mg/L soak for 20 min,rooting powder 800 mg/L soak for 15 min and Guoguanggenpan for dilution 200 times and then for 5s,the treated cuttings are inserted in the sand bed in the orange garden of the West Campus of Yangtze University.Each experment was repeated 3 times,and the data was collected after 30 days.In the March 2017 trial,it was found that the cuttings of the cuttings had neither callus formation nor rooting;in June 2017,it was found that only callus was produced in the cuttings,and the callus induction rate was 3.67%;In the January 2018 test,callus and conical small bulges were produced in the cuttings of the cuttings in March,and the callus induction rate was 6%.The results showed that the cuttings of C.duclouxii had no significant effect on cutting results in different seasons（P>0.05）.Different exogenous growth regulators had significant effects on the callus induction rate of C.duclouxii cuttings（P≤0.05）,and the best effect was obtained after soaking rooting powder 200 mg/L for 1h.3.In September 2017,according to the 10 treatment methods for the C.duclouxii cutting base in June 2017,the c.serrulata var.lannesiana‘Sekiyama’cuttings cultivated locally in Jingzhou City were treated,and then the cutting experiment of C.serrulata var.lannesiana‘Sekiyama’was carried out in river sand and loam.The results showed that under the treatment of different exogenous growth regulators,the C.serrulata var.lannesiana‘Sekiyama’cuttings were able to produce adventitious roots in both matrices.The best rooting effect was obtained by soaking in IBA 50 mg/L for 2h and IBA100mg/L for 1h.In loam soil,the rooting rates of IBA50 mg/L and IBA100 mg/L were56.67%and 50%,respectively.In river sand,the rooting rates of IBA50 mg/L and IBA100 mg/L were 46.67%and 53.33%,respectively.Different exogenous growth regulators had no significant effect on the rooting of C.serrulata var.lannesiana‘Sekiyama’（P>0.05）.Rooting rates of C.serrulata var.lannesiana‘Sekiyama’cuttings treated with water in loam and river sand were 15%and 30%respectively,indicating that C.serrulata var.lannesiana‘Sekiyama’cuttings were easier to take root.Different matrices had a significant effect on the rooting effect of C.serrulata var.lannesiana‘Sekiyama’（P≤0.05）,and the C.serrulata var.lannesiana‘Sekiyama’cuttings were better with river sand matrix.4.In order to explore the effect of Arbuscular mycorrhiza fungi on the cutting of C.duclouxii branches,the cutting experiment was carried out with C.duclouxii branches in August 2018.The cuttings are treated by four different treatments:rooting powder 200mg/L soaking for 1h,rooting powder 500 mg/L soaking for 20 min,rooting powder 800mg/L soaking for 15 min and Guoguanggenpan for diluting 200 times and then for 5s,cutting in mixed with branches In the river sand matrix plastic pots of AMF,the control was not inoculated.After 30 days,the callus was observed at the part of the incision,and the white point-like bulges appeared at the base of the individual cuttings.The callus induction rate was the highest after the treatment with 200 mg/L of raw root powder,which was 18.46%.The callus induction rate of C.duclouxii was not significantly affected by the treatment with different concentrations of raw root powder and inoculation with AMF（P>0.05）.5.The buds of C.duclouxii,C.yedoensis,and C.serrulata var.lannesiana‘Sekiyama’were used as explants.The stems with buds were soaked in detergent water for 20 min,the surface of the branches was brushed and rinsed under running water for30 min,transferred to the clean bench,and disinfected with 75%alcohol for 1 min,then 0.1%mercury for 10 min,and finally washed with sterile water 5 times.After the surface water of the scale buds was evaporated,cut the disinfected scale buds with a scalpel and take out the buds from the center of the scale buds into MS medium.The sterility of C.duclouxii and C.yedoensis was 18.45%and 12.31%,and the sterility rate of C.serrulata var.lannesiana‘Sekiyama’was 75.31%,which was significantly higher than that of C.duclouxii and C.yedoensis（P≤0.05）,indicating that it is not easy to establish a high-quality tissue culture system for cherry scale buds.6.At the induction stage of callus,the tissue buds of C.duclouxii and C.yedoensis were randomly transferred into callus induction medium:MS+GA₃0.2 mg/L+6-BA0.5 mg/L+NAA0.05 mg/L,The callus induction rates were 61.67%and 79.6%,respectively.The C.serrulata var.lannesiana‘Sekiyama’group buds were equally transferred to three proliferation media:MS+6-BA0.5 mg/L+NAA0.2 mg/L,MS+6-BA1.0 mg/L+NAA0.2 mg/L,MS+6-BA0.5 mg/L+GA₃0.2 mg/L,the callus induction rate of C.serrulata var.lannesiana‘Sekiyama’cherry blossom is 100%.In the rooting culture stage,the rooting rate of C.duclouxii on 1/2MS+NAA0.1 mg/L+IBA0.5 mg/L medium was 1.67%,and the rooting of C.yedoensis on 1/2MS+IBA1.0mg/L medium The rate was 2.04%,and the rooting rate of C.serrulata var.lannesiana‘Sekiyama’was 1.75%.Three kinds of cherry blossoms are more likely to induce callus,but it is difficult to induce rooting of sterile seedlings.7.In March 2016,Forestry Research Institute of Shennongjia introduced 35 strains of C.yedoensis‘Yedoensis’and 20 strains of C.serrulata from Wuhan University,and planted in Qianjiawan Village of Yangri Town.To explore the adaptability of these two introduced cherry blossoms,the soil and climatic conditions of the introduction of cherry blossom native land and introduction land were compared and the relative growth of the introduced cherry blossoms was investigated.The results showed that the climatic environment and the soil condition in the Shennongjia Forestry District were suitable for the growth of cherry blossoms.After one year of growth,both cherry blossoms can survive and grow.The growth of C.serrulata is better than C.yedoensis‘Yedoensis’.In general,the introduction of cherry blossoms has a good adaptability in the Shennongjia Forest District,and can further introduce other varieties of cherry blossoms.