Research On Key Techniques Of Tissue Culture And Cutting Propagation In Cerasus Humilis(Bge.) Sok

This study focused on the problem of tissue culture and cutting propagation in the case of Cerasus humilis(Bge.)Sok.the’Jingou No.2’ and’Gaiguo No.6’ were used as test materials to improve the Cerasus humilis tissue culture system.The rooting morphology of Cerasus humilis tissue culture seedlings were observed by microscopic observation.The endogenous hormones(GA3,IAA,ABA),flavonoids,total phenolics and sugar content in the four types of seedlings were compared and analyze d.The effects of different greenhouse light supplementation conditions on cutting seedlings of Cerasus humilis were analyzed.Furthermore,it provides a reference for tissue culture and cutting propagation techniques of The main findings are as follows:(1)Established the organizational culture system of Cerasus humilis:The best disinfection method for Cerasus humilis explants is 10%sodium hypochlorite solution for 10 minutes.The optimal primary culture medium for ’Jingou No.2’ is the MS+0.5 mg/L 6BA+0.1 mg/L NAA,and the optimal proliferation medium is MS+0.5mg/L6BA+0.1mg/LNAA,the best rooting culture The base is 1/2MS+0.7mg/LIAA,and the optimal growth rate of ’Gaiguo No.6’ is the MS+1.0mg/L6BA+0.07mg/LNAA.The optimum rooting medium is 1/2MS+0.7mg/LIAA+0.15 mg/L NAA.(2)Through microscopic observation of rooting process of tissue-cultured seedlings of Cerasus humilis,it was found that the cambium divides and the pith widens at 6th days of growth,and meristematic tissues can form at 9th days of growth,then root primordia can be formed.Finally,rooting can be formed through vascular bundles.This indicates that tissue-cultured seedlings of Cerasus humilis belong to induced rooting type,and their development process may affect rooting rate.In the stem and root of the Cerasus humilis,the proanthocyanidins are distributed in the vascular bundle and the medullary ray.And with the formation of new vascular bundles,the color deepens.(3)①The content of GA3 in roots(12.164 ± 0.23 mg/g)with normal seedling(R-G,no callus-rooted-new leaves)was significantly lower than that of other types of seedlings.②The content of IAAin normal leaves and old leaves(12.164±0.23 mg/g)was significantly higher than that in other types.In the cuttings with callus,the IAA content of rooted seedlings was significantly higher than that of rootless seedlings.③The ratio of ABA/GA3 and(IAA+ABA)/GA3 in normal seedlings and old leaves was significantly higher than other types.④The total phenolic content of root cuttings in roots and old leaves was significantly higher than that of rootless types,and the content of new leaves in normal seedlings was very high,which was 0.642±0.01 mg/g.⑤The content of flavonoids in the roots of root cuttings was significantly higher than that of rootless type,but there was no significant difference between the content of flavonoids in normal seedlings and other types..⑥The total sugar content of cuttings with callus formation(callus,annual stem and old leaves)was significantly higher than that of no callus,while the root-type cutting seedlings had significantly higher glucose content in roots than without roots.The content of callus.(4)①The fresh and dry weights of cuttings treated with BR(supplemented red and blue light)were 2.94 and 2.46 times higher than those of the control,respectively.②W(white light supplementation)and BR treatment could significantly increase shoot length,diameter,number of leaves and survival rate.③The total phenol content in roots and annual stems treated with BR and W was significantly lower than that of CK(0.546±0.01 mg/g、0.623±0.02 mg/g).The total phenol content in new stem and leaf was significantly higher than CK(0.324±0.01 mg/g、0.227±0.00 mg/g).④In roots,annual stems,and new stem segments,the total sugar content of W treatment was significantly higher than that of CK and BR,while in leaves,BR and W treatments were significantly lower than the total sugar content of the control.