| Cherry blossoms are ornamental plants from Rosaceae family Prunoidea Subfamily and Creasus genus.In recent years,with the rapid development of the cherry blossom boom,the demands for cherry blossoms in the market have increased year by year.The traditional grafting methods of cherry blossom can’t meet the demands of the market anymore.The culture and rapid propagation of cherry blossoms are still to be further explored to meet the demands,which will develop the industry and economy of cherry blossoms in China.This paper mainly researches on tissue culture,rapid propagation technology,the induction of embryogenic callus of nine different species of cherry blossoms.The chemical regulation between hormones has a significant effect on them.This paper mainly researches on the rapid propagation of tissue culture of several Chinese cherry species such as Cerasus discoidea,Cerasus pilosiuscula and Cerasus conradinae,and several cultivars such as Cerasus campanulata×kanzakura‘Kawazu-zakura’,Cerasus campanulata×kanzakura‘Rubescens’and Cerasus×yedoensis‘Somei-yoshino’,and aromatic cherry varieties Cerasus serrulata var.lannesiana‘Sirotae’and a kind of Japanese cherry rootstock.In addition,Cerasus pilosiuscula and Cerasus conradinae II were seleted to study their embryogenic callus induction.The effects of different genotypes,different combinations of hormones,and concentration ratios on rapid propagation and somatic embryogenesis were studied.The main contents and conclusions are as follows:（1）Taking nine different kinds of cherry blossoms as the research object,with their axillary bud stem segments as experimental materials,the effects of plant hormone combinations and concentration ratios on sprouting,proliferation,and rooting of different cherry species were studied.The results showed that the time of mercury disinfection of explants controlled between 10 and 12 minutes could ensure the survival rate of the aseptic seedlings.The germination rates were above 59.26%and the Cerasus pilosiuscula was up to96.30%when the explants were inoculated with MS+6-BA 1.0 mg/L+NAA 0.1 mg/L or MS+6-BA 1.0 mg/L+IBA 0.1 mg/L.The medium which is good for the growth of Cerasus pilosiuscula,Cerasus conradinae I,Cerasus discoidea and the Japanese cherry rootstock is MS+6-BA 1.0 mg/L+NAA 0.2 mg/L;MS+6-BA 1.0 mg/L+NAA 0.1/0.2 mg/L+GA3 2.0mg/L is more suitable for the proliferation of Cerasus conradinae II.The medium which is good for the growth of Cerasus campanulata×kanzakura‘Kawazu-zakura’is MS+1.0mg/L 6-BA+0.1 mg/L NAA.The best method for the several cultivars and aromatic sakura varieties Cerasus serrulata var.lannesiana‘Sirotae’,Cerasus campanulata×kanzakura‘Rubescens’and Cerasus×yedoensis‘Somei-yoshino’is to culture in the medium MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+GA3 5.0 mg/L for 15 days then transfered to the medium MS+6-BA 1.0 mg/L+2,4-D 0.2 mg/L.The highest proliferation coefficient is 5.67.When rooting,medium 1/2 MS+IBA 0.5 mg/L and 1/2 MS+NAA 0.5 mg/L were all suitable for the rooting culture,and the rooting rate was up to 100%.The rooted seedlings were transplanted to a culture medium with V（vermiculite）:V（perlite）:V（peat soil）=1:1:1,and the survival rates were all over 80%.The young leaves of the progeny shoots of Cerasus pilosiuscula and Cerasus conradinae II were used as test materials to study the effects of factors such as plant hormone combinations and concentration ratios on embryogenic callus induction.The results showed that the leaves of Cerasus pilosiuscula and Cerasus conradinae II were inoculated into medium MS+KT 1.0 mg/L+2,4-D 2.0 mg/L+NAA 0.5 mg/L for 15 days respectively,then transferred to the medium MS+1.0 mg/L KT+0.5 mg/L NAA without 2,4-D for 40days.The embryogenic callus differentiation rates of Cerasus pilosiuscula and Cerasus conradinae II were high,which were 32.5%and 31.5%,respectively. |
