Study On Rapid Propagation Of Cuttage And Tissue Culture Technology Of Ampelopsis Grossedentata

Seed propagation of Ampelopsin Grossedentata leads to inconsistent progeny characters,and it takes a long time for seedlings to grow for yield.Therefore,it is mostly used for variety breeding but not suitable for field production.Cutting breeding of Ampelopsin Grossedentata results in consistent progeny characters,and only a short time is required for seedlings to grow for yield.Therefore,cutting breeding is mainly adopted in production.However,it is associated with low survival rate of the cutting seedlings and reduced yield after years of planting,which requires tissue cultured seedlings for rejuvenation.This paper carried out research on cutting breeding technology and tissue cultured seedling rapid propagation technology of Ampelopsin Grossedentata targeting the existing practical probleMS in production of Ampelopsin Grossedentata.The major research achievements were shown as follows:Firstly,treating the annual branch of Ampelopsin Grossedentata with 20mg/kg IBA during ordinary cutting resulted in optimal length of adventitious root,number of shoots,height growth,biomass and survival rate.Consequently,20mg/kg IBA could be adopted to treat the annual branch of Ampelopsin Grossedentata in production for cutting propagation.Secondly,stem leaf growth,total root length,biomass and strong seedling index in two-stage cutting breeding were all higher than those in conventional cutting breeding;therefore,two-stage cutting breeding was recommended during field production if time and field conditions permitted.The best matrix of level 1 seedbed in two-stage cutting breeding was peat soil: river sand= 1:1.Thirdly,the optimal primary medium in rapid propagation system of tissue cultured seedling of Ampelopsin Grossedentata was MS+1.5 mg/L BA+0.2mg/L NAA+4 g/L PVP.The optimal matrix matching for primary stem cultured callus was MS+4.0 mg/L BA+2.0 m/L ZT+1.0 mg/L NAA.The optimal proliferation medium for Ampelopsin Grossedentata bud was MS+2.0 mg/L BA+1.0 mg/L NAA+2.0 mg/L ZT.The optimal strong seedling medium was MS+1.0 mg/L BA+1.0 mg/L NAA.The optimal rooting medium was NAA(0.1 mg/L)+IBA(0.5 mg/L),with the rooting ratereaching 86.7%.The optimal transplantation matrix at hardening-seedling stage was peat + perlite(2:1),and the hardening-seedling and transplanting survival rate could reach 73% if it was watered with modified Hoagland nutrient solution.Fourthly,it could be observed when comparing contents of major effective components and activity of antioxidant enzyme(SOD)among three reproductive patterns that,contents of flavone and polyphenol in tissue cultured seedling of Ampelopsin Grossedentata were lower than those in cutting seedling,while polysaccharide content was higher than the latter,and SOD activity was slightly lower than the latter.Two-stage cutting seedling had higher contents of effective components and SOD activity than ordinary cutting seedling,and difference in flavone content was statistically significant.