| Hepatic glucose metabolism plays an important role in the performance and health of the dam.Insulin inhibits glucose synthesis in liver.Niacin can affect the body's energy metabolism through various pathways.Based on the hypothesis that adding nicotinic acid and altering the dietary crude-to-coarse ratio can affect the hepatic glucose metabolism in perinatal sheep,this study explored the effects of dietary induction on hepatic glucose metabolism,insulin signal transduction and the rate-limiting enzyme gene expression under the p53-SIRT6-Fox01 axis.The following experimental studies were conducted.The experimental research in this paper is divided into two parts:Part 1: Effect of Different Rough Crude Ratio Diets on Hepatic Glucose in Perinatal EwesThirty mortalities of 56.41±4.24 Kg perinatal 3-year-old Erdos fine wool ewes were randomly divided into 3 groups with 10 animals in each group.Namely: control group,group 1th(rough ratio 2:8 group)and 2th group(rough ratio 4:6 group).The herdsmen of the control group were fed according to the original feeding program.The ewes of the experimental group were supplemented at 7:00 in the evening to prepare the concentrate and freely drink water.The experimental prefeed period was 15 days and the normal feeding period was 40 days.The indicators of production performance,blood physiochemical propertie,liver gluconeogenesis key gene expression and protein expression were determined.The results showed that there was a significant difference in weight of ewes at 21 days postpartum(P<0.05).The weight of the ewes in the second group was significantly higher than that of the control group.There was no significant effect of different diets on milk yield and milk composition(P>0.05).There was a significant difference in plasma concentrations of NEFA and INS between ewes during the perinatal period(P<0.05),Compared with the control group,GLU concentration in plasma of the second group was significantly higher,while NEFA and INS concentrations were significantly lower.Compared with the control group,m RNA abundance and protein activity of PCK1,G6 P,Fox01,PI3 K,AKT,and GLU2 were lower in the liver of the experimental group,while mRNA abundance and protein activity of p53,SIRT6 and INSR were higher.INSR-AKT/PI3K-Fox01 pathway activates Fox01 protein activity and regulates PCK1 and G6 P activity and further regulation of gluconeogenesis.Part II: Effect of different diets NFC/NDF and nicotinic acid supplementation on hepatic gluconeogenesis in perinatal ewesForty mortalities of 58.9±4.55 Kg perinatal 3-year-old Erdos fine wool sheep ewes were randomly divided into 4 groups with 10 animals in each group.With a 2×2 completely randomized trial design,the first factor was the ratio of NFC to NDF of 0.44 and the ratio of NFC to NDF of 0.84.The second factor was the amount of coated niacin added to the diet of 2 g/d and 4g/d each,constitute a total of 4 groups of diets.That is,Group I(NFC/NDF = 0.44 + 2g),Group II(NFC/NDF = 0.44 + 4g),Group III(NFC/NDF = 0.84 + 2g)and Group IV(NFC/NDF = 0.84 + 4g).The test period is from 40 days before delivery to 21 days after delivery.The stability of non-degraded niacin in the rumen was tested and ewes performance,blood physiochemical properties,expression of key hepatic gluconeogenesis genes,and protein expression were measured.The results showed that the solid palm oil coating material can protect NA,and the release rate of NA in artificial saliva and the rumen of the body is lower,the stability is better,and the release rate is higher in the duodenum.The amount of coated niacin added to the diet of 4g/d each,the concentration of NEFA in the perinatal blood of ewes can be significantly reduced and NEB can be reduced.AKT/PI3K-Fox01 pathway can be regulated by INS signal transduction to regulate gluconeogenesis.NAH+ regulates the p53-SIRT6-Fox01 pathway and regulates Fox01 protein activity,which in turn regulates gluconeogenesis. |
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