Study On The Difference Mechanism Between Two Varieties Of Rice To Transfer Selenium Into Grain

Selenium is a necessary trace element in human beings and animals,although there is no plants that selenium is necessary nutrition elements,but the amount of selenium can promote plant growth and development,food is the main way to intake of selenium in human body,the content of selenium in soil and its determining the level of food containing selenium.However,according to the statistics,there are 72% of the area,about 700 million people in a state of lack of selenium,restricts the normal demand of selenium,and the body is the main source of selenium food,and most human consumption in the world the most grains,rice and processing,transportation and storage is convenient,safe and efficient to the human body for selenium selenium content in rice nutrition has important significance.Therefore,it is very important to increase the selenium content in rice grains.For more than 30 of the conventional rice selenium-rich study found that under the same level of selenium,there were no significant differences in yield,feng accounted for eight and grain selenium levels of two rice varieties two times,so as a breakthrough to explore the differences between two varieties of rice selenium transport,in order to improve the rice level and gradually improve human selenium content of selenium intake provided the scientific basis.1.Compared with the non selenium treatment,the effect of Selenium Application on the growth and development of rice began to appear after 42 d.In the 64 D-72 D period,the plant height of high accumulative rice after selenium application was significantly higher than that of low accumulation of.5mg·Kg-1.The selenium application had a significant effect on the fresh weight and yield of the two rice fields.The relative chlorophyll content(SPAD)and aboveground fresh weight of rice leaves were significantly higher than those of low accumulative varieties,but there was no significant difference in grain yield.2.Soil selenium significantly increased the selenium content and organic selenium content in the brown rice,and the total selenium content and the organic selenium content of the high cumulative rice grains were significantly higher than those of the low accumulative varieties at the same level of selenium.The selenium content of rice husks and stems in the high accumulative Rice Cultivars was slightly higher than that of the low accumulated varieties,while the two varieties of rice leaves and roots were also higher than those of the low accumulated varieties.Selenium content was almost no difference.Soil selenium significantly increased the migration coefficient of selenium in rice root and stem,leaf grain,and the migration coefficient of selenium in the root and stem of high accumulated water in the same level of selenium at the same level of selenium,and the migration coefficient of selenium in the stem and leaf was the opposite.Organic selenium was the principal in the brown rice.The form of selenium is about 72.2 + 3.3% of all selenium,and the proportion of organic selenium in brown rice has no significant difference between different rice varieties in different selenium treatment or under the same level of selenium application.3.With no significant difference in selenium content,under the selenium level of 5.0mg·kg-1 at the early stage of grain filling,the proportion of the partial selenium in the residue of low accumulated varieties increased by 15.72%,and the proportion of the soluble portion of the high accumulated variety was 15.85% more than that of the low accumulation.At the late stage of grain filling,the distribution ratio of the residue fractions of low cumulative ratio and high accumulation varieties decreased by 14.03% under the level of 1.0mg kg-1 selenium application,and the low accumulation ratio of the soluble portion was reduced by 14.01% in the high accumulation rice,and more available selenium in the high accumulative varieties.4.As the amount of selenium increased,the selenium content of rice spikes increased gradually at the grain filling period,and there was a significant difference between the spikes and the sword leaves of two rice varieties under the level of 5.0 mg·kg-1 selenium,indicating that the high accumulative varieties could absorb and transport selenium better;and the selenium content of two varieties of rice under the level of 0.5,1.0 mg·kg-1 at the middle of the filling stage There was no significant difference between them,probably due to the dilution effect in the filling stage.5.In the middle grain filling period,the nitrogen content of the high accumulative varieties increased with the increase of selenium content,and there was no significant difference in the ear nitrogen content in other periods,and the nitrogen content of the low accumulative varieties at the early stage of grain filling decreased,and there was no significant difference in the phosphorus content of the two varieties at the early filling stage.At the middle and late filling stages,the phosphorus of the high accumulated varieties reached the lowest level in 1.0 mg·kg-1 and was significantly different from the other two selenium treatments.In the middle of the filling period,the potassium content of the high accumulated varieties of the sword and rice spikes were lower than those of the low accumulative varieties.The potassium content of the two varieties of rice spikes at the late filling stage were all along with the amount of selenium applied.The accumulation of potassium in flag leaves decreased with the increase of selenium content.6.During the filling stage,the content of Ca,Mg,Fe,Mn increased with the growth period,but the content of Cu decreased with the increase of growth period.As the amount of selenium increased,the content of Ca decreased,and the content of Mg,Cu and Mn was not changed,but Fe was raised first and then decreased,and no significant changes were found at other stages.During the filling period,the content of Ca,Mg,Fe,Mn,Cu increased with the growth period.As the amount of selenium increased,the content of Ca,Mn,Cu decreased,and the content of Mg did not change,while Fe first increased and then decreased.7.Without application of selenium,the relative content of each transporter did not change significantly after selenium was added to the low-accumulation cultivars,but the high accumulation reached SULTR1;2 at 2h;the relative expression of SULTR2;1 was the highest,and SULTR2;1the relative expression of 1 decreased with time and then increased,while the relative expression of the relative expression of SBP1 was over the time.In the case of selenium,the relative expression of the high accumulative variety was significantly higher than that of the low accumulated variety.The relative expression of the high accumulative SBP1 was significantly higher than that of the low accumulative variety under the condition of selenium application,and the relative expression of the high accumulative variety SBP1 was significantly higher than that of the low accumulative variety under the condition of selenium application.The relative expression of the high accumulation of SBP1 was higher than that of the low cumulative varieties.The relative expression of high accumulation SULTR1;2 was increased and the relative expression of SBP1 decreased after adding selenium among the same varieties.