| 22 kinds of elements are needed to maintain the human body, Selenium is one of the necessary elements, the lack of Selenium has a positive correlation relationship with diseases such as Kashan disease?muscular dystrophy and esophageal cancer. People can mainly intake Selenium from diet, compared to that of animals, the Selenium relative content of plant sources dietary is low, but the wheat is the main dietary source of human, so the Selenium content of grain is closely related to health. To carry out the breeding of rich Selenium of wheat is one of the most economic and effective way to overcome the lack of Selenium, so need to research and identify the gene of control the grain Selenium content.Wheat is one of the Selenium non-sensitive plant and the Selenium content of wheat grain is generally low. A high selenium content wheat materials derived from synthetic SHW-L1 which grain kernel Selenium content is higher than common wheat 4 to 9 times. selenium content and root morphological quantitative traits of wheat are quantitative character Understanding the underlying genetic basis of selenium contentseed composition is crucial to improving seed selenium. In this study we used recombinant inbred line mapping populations derived from SHW-L1 and constructed a joint linkage map from the population. The major study and result were described as follows:1. Agronomic trait statistics such as plant height, the tiller number, anthesis of the recombinant inbred lines (SHW-L1×Chuanmai32 RIL F9) of 171 wheat genetic groups were tested for two years. The result showed that the addition of selenium can increase the plant height, significant young flowering in advance and reduced tiller number, variance analysis showed that the agronomic traits of the genetic groups significantly influenced by the Selenium levels.According to the builded genetic map, a total of 24 QTLs were identified in present study, which explained 5.42-30.41% of the phenotypic variation for two years in a row in 2013 and 2014 at Wen jiang.2. The Se content in RIL lines and parents were analyzed of 171 RIL lines and the results indicate that parents SHW-L1 were higher than Chuanmai32 in two years. QTL mapping for selenium content was performed through the high resolution map of SC population. A total of 6 QTLs were identified in present study, which explained 17.13-80.16% of the phenotypic variation and distributed on the chromosome 3D?5A. especially QTLs on the chromosome 3D (wPt-733447-wPt-667315). especially the major locus for selenium content on 2D, which also showed major effect on the selenium content of wheat kernal. These environments independent QTLs should be great value for yield breeding in wheat.3. The Hydroponic experiment of wheat seedling were treated under the low (0.05ug/g) and high concentration (0.5ug/g) for 6-9 weeks, AVONA variance analysis showed that the root traits were affected by for selenium levels significantly. A total of 245 QTLs were identified in the population, which explained 5.42-30.41% of the phenotypic variation,67 of which explained the phenotypic variation higher than 10%, and 7 of which were detected in all investigated environments.32 QTLs had multiple effect such as the QTL sites that controlled the total projection area (PA) which was identical with the QTLs about the total area (SA), root volume (RV).4. The concentration of selenium has a significant impact on the transfer of nutrient from the soil to the over ground parts of wheat, thus affecting dry weight, fresh weight and root morphology. As the cultivate time increases, selenium transport rate from the underground part to the ground was declined. The rule of the accumulation of selenium in plant is root> stem> grain.According to the builded genetic map, QTL mapping for selenium content in root and aboveground was performed under the conditions of hydroponic using software WinQTL2.5.38 QTLs were detected.2 QTLs are expressing in different environments. Selenium content control sites changes as the training cycle different. |
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