Asthma Drugs Validation Based On Humanized Mice And Research On Drug Recovery Of Graphene Oxide

Asthma is a common respiratory immune disease in children and adults.Its pathogenesis is mainly the imbalance of th1 and th2-related cytokines.Interleukin 4(IL-4)is an inflammatory cytokine that plays an important role in the pathogenesis of asthma.Therefore,we use gene editing technology to replace humanized IL-4 and IL-4RA proteins with corresponding mouse-derived proteins,respectively,to establish double humanized IL-4/IL-4RA(hIL-4/hIL-4RA)mouse model,and prepare asthma model to achieve the purpose of screening antibody drugs.The modified Hummers method is used to synthesis graphene oxide(GO),and the film made of it is used to study the recovery rate of fluorine-containing drugs.The main research results are as follows:1.We performed phenotype analysis and identification of hIL-4/hIL-4RA mice by Southern blotting,ELISA and flow cytometry.The results showed that hIL-4/hIL-4RA mice can express humanized IL-4 IL-4RA protein,and verified that its downstream pathway is not blocked.2.The ovalbumin(OVA)induced hIL-4/hIL-4RA mice showed important characteristics of asthma such as inflammatory cell infiltration,IgE release,and th2 cytokine secretion.In addition,treatment of these humanized mice with anti-human IL-4RA antibodies can significantly inhibit the levels of these pathological indicators.Therefore,hIL-4/hIL-4RA mice provide a validated preclinical mouse model that can be used to study new therapeutic agents targeting this specific cytokine pathway in asthma.3.Initially explored the recovery rate of fluorine element in NaBF4 by GO and rGO.