Research On Refractive Index Decoupling Method Of Multimedia Cells Based On Quantitative Phase Micromorphology Reconstruction

Cells are the basic structural units composed of various living bodies,and are the basic units of life activities.They play the functions of absorbing energy,transmitting information and differentiation in the living bodies.Its size,morphology and internal structural characteristics are closely related to the health status and physiological activities of cells,so the microscopic imaging technology of blood cell morphology has played an important role in biological sciences and clinical medical applications.In microscopic imaging technology,the optical phase detection method,with its high imaging accuracy,real-time imaging,and quantitative analysis of experimental data,has shown unique advantages that traditional detection methods do not have,and has become one of the important methods of cell detection analysis.The phase obtained by the phase microscopy technique reflects the integral of the sample refractive index and the thickness along the optical axis.Therefore,there is a coupling between the refractive index and the thickness.To reconstruct the three-dimensional shape of the cell,it must be decoupled.Most of the current refractive index decoupling methods are proposed for homogeneous cells.Due to the complex internal morphological structure of heterogeneous cells,there is no perfect method and theory to decouple the cell thickness and internal refractive index distribution information from the phase distribution.In order to realize the three-dimensional reconstruction of multi-media and sub-structure cells,based on the traditional phase microscopy technology and single-medium refractive index decoupling theory,this paper has carried out the research on the refractive index decoupling method of multi-medium cells.The research content is as follows:1.Comprehensive analysis of various phase microscopic imaging methods and gradient edge detection algorithms,and a method of quantitative phase microscopy combined with gradient operator to decouple the refractive index of multi-media cells.In this method,only two phase diagrams in orthogonal directions can be used to achieve the decoupling of the refractive index,and the simulation experiment results based on the MATLAB simulation platform and the physical experiments of real lymphocytes are carried out.The effectiveness of the method.2.Aiming at the problem that the shape of heteromorphic nuclear cells is complex and the shape of nuclei is difficult to fit,based on the traditionaldual-wavelength decoupling refractive index method,the refractive index of heterogeneous nuclear cells is decoupled.A series of simulation experiments were carried out based on the MATLAB platform,and the experimental results all showed the applicability of this method in decoupling the refractive index of heteromorphic cores.The research in this paper has made some progress in the decoupling of refractive index information of multi-media blood cells,which provides a new method for the decoupling of multi-media cell refractive index.It provides a new technical basis for the development of three-dimensional microscopic imaging technology of multi-media blood cell substructure.