The Weak Absorption Cell Sample Phase Quantitative Measurement Technology Research Field

It’s an effective way to obtain the distribution of the internal structure of the biological cell samples by means of Diffraction phase microscopy.But existing methods ignored the existence of the cell weakly absorbing and imaging beam unevenness, which affects the accuracy of quantifying cell structure.To this end,we proposed a method to correct the cell weakly absorbing and imaging beam unevenness in this thesis, and this method can be practical application.This thesis introduced the research status at home and abroad of the Diffraction phase microscopy,and its basic principle and the typical implementation method. On this basis, we established a Diffraction phase microscopy system as the experimental system for quantifying cell structure.As we have put forward a method to correct the cell weakly absorbing and imaging beam unevenness,we applied this method to Fourier phase extraction algorithm and Hilbert phase extraction algorithm,and proved it is feasible and practical. Combined with the known information,we create a simulated interferogram and reconstructed the phase distribution of the red blood cell using the matlab, and the results show that the correction method improved the accuracy of the measurement obviously,and improved the degree of fit of the reconstruction results with the original results from0.9683(absorption coefficient3.5,HT) up to0.9989compared with before the correction. During the experiment,we extracted the red blood cells of the rabbit by centrifugation and obtained clear interference images.We reconstructed the2-D phase distribution of the cells through Fourier phase extraction method, Hilbert phase extraction method and the corrected method, and compared and analyzed them. Fully visible,this research has a certain reference value for the optimization of the Diffraction phase microscopy.