Distribution Of Arbuscular Mycorrhizal Fungi In Rhizosphere Soil Of A Uranium Mining Area And Its Relationship With Soil Factors

With the progress of uranium mining in China,the problem of treatment and repair of radioactive uranium pollution in decommissioned uranium mines immediately.The radioactive pollution in uranium mining area has the characteristics of wide pollution area and difficult management.Although traditional physical and chemical methods have shown good repairing effect,it is difficult to apply to pollution control of uranium mining areas with large pollution scope.More scholars are responsible for its restoration and treatment.Focus on low-cost,environmentally friendly phytoremediation technologies.Related studies have shown that phytoremediation technology has a certain effect on the repair of radioactive uranium pollution,but its anti-pollution tolerance makes the repair effect limited.However,the symbiont-arbuscular mycorrhiza formed with plant roots has enhanced plant antipollution properties,and it is expected to use it to enhance plant anti-pollution tolerance and improve the effect of repairing radioactive uranium pollution.This paper selects the uranium mining area and tailings reservoir soil area in a mountainous area in the south as the research object,and explores the distribution characteristics of uranium pollution in the study area,the resource distribution of arbuscular mycorrhizal fungi(AMF),AMF and soil factor and Correlation of uranium content.The research results have been the preliminary work of the arbuscular mycorrhizal technology to control the uranium pollution in the mining area.The main research results are as follows:(1)The uranium mining area and the uranium tailings reservoir area have been seriously polluted by uranium sampling in the study area.Among them,the most serious pollution in uranium mining area is the soil around two mines and landfills,and the change of uranium concentration in soil is greatly affected by the change of topography;the most serious pollution in uranium tailings reservoir area is the soil in tailings reservoir,and the change of uranium concentration in farmland soil downstream of tailings reservoir is mainly affected by irrigation water.The content of uranium in rhizosphere soil was slightly higher than that in surface soil,indicating the possibility of enrichment and fixation of uranium in soil by plants.(2)Through investigation and identification,11 typical plants were collected in the study area,and 5 genera(Bacillus,Acute Mycocystis,Bacillus constricta,Mycocystis,Rhizocystis)were isolated from the root and rhizosphere soil of the plants.12 species of arbuscular mycorrhizal fungi were identified.Among them,only 9 AMFs were found in tailings reservoir area,while 12 AMFs were found in uranium mining area.The infection rate of AMF ranged from 26.64% to 77.21%,with an average of 47.52%.The spore density in rhizosphere soil ranged from 84 to 112 per 25 g air-dried soil,with an average of 100.2.(3)The correlation between soil AMF,Ballomycin and soil factors in uranium mining area and tailings reservoir research area was analyzed by SPSS software.Available nitrogen,available phosphorus and pH in soil were correlated with AMF infection rate,spore density and sacculomycin content in rhizosphere soil.The correlation between uranium in rhizosphere soil and AMF infection rate in rhizosphere soil,spore density and sacculomycin content in rhizosphere soil was not significant,indicating that AMF was also found in uranium-contaminated soil.It can grow normally.According to the analysis of soil factors in the study area,available potassium,available phosphorus and alkaline nitrogen are the key factors affecting the distribution of AMF.