Biodiversity And Distribution Of Arbuscular Mycorrhizal Fungi(AMF) And Other Soil Microorganism Of Aeolian Sandy Coal Mining Subsidence Area

The coal mining subsidence result in soil erosion,land productivity decline,destruction of surface drainage system,soil biodiversity variation,degradation and desertification of grassland.The symbiosis between arbuscular mycorrhizal fungi and plant roots is common in natural ecological system.AM fungi play an important role in the regulation of nutrition of plant roots and soil.The diversity and distribution of AMF were studied by the morphology and molecular method.The AM mycorrhiza characteristics of common plant,and the relationship between the AM fungi with the soil chemical property,soil microbial factors were investigated in the ShenDong coal mining area in Inner Mongolia.The main results are as follows:1.There are 27 species belong to 9 genera isolated and identified from AM fungi spores in research area,and 4 AM fungi species are belonge to Acaulospora(2 unknown species),1 species belong to Entrophospora,2 species of Claroideoglomus,2 species of Diversispora,1 species Scutellospora.13 species Glomus,Funnelifomis,Rhizophagus,Septoglomus of Glomeracea,and 4 unknown species.11 and 12 species belong to 5 and 7 genera were isolated in the crack and non fracture area respectively.14 and 14 species belong to 6 and 8 genera were isolated in higher or lower disturbance intensity area respectively.Septoglomus constrictum,Glomus convolution,Funnelifomis mosseae were the dominant species in the study area.2.There are difference in diversity of AM fungi including spore density,abundance and diversity index among differ area.The spore density of AM fungi was significantly higher in non fracture zone than fracture zone(P<0.05).The spore density of AM fungi of higher disturbance intensity sample area was significantly higher than that in the lower disturbance intensity(P<0.05).3.More than 80%of plant roots were infected by AM fungi in coal mining subsidence area.There are difference betwceen the plant species,and the infection rate of perennial plants is higher than that of annual plants.4.The molecular diversity results showed that the OUT of AM fungi belongs to Diversispora,Glomus,Funneliformis,Paraglomus,Ambispora,Sscutellospora,Claroideoglomus,Septoglomus.The AMF genus number is higher than the morphological type.And the OTU AM fungi number are higher in non fracture zone and lower disturbance intensity sites than fracture zone and higher disturbance intensity,respectively(P<0.05).5.Total soil fungi numbers are 0.87-12.50×103 ?·g-1 soil,and bacteria number are 0.95-7.00×107 ?·g-1 soil.The microbial biomass carbon and nitrogen are 146.68-988.46 mg·kg-1 and 27.55-116.17 mg kg-1 respectivrly.The soil fungi and bacteria biomass were decreased with the deep of soil layer,and significantly correlations with the fracture and disturbance intensity(P<0.05)?6.The species and conponent of soil microorganism community were checked by high-throughput sequencing analysis depend on the 16S rRNA sequence.The study found different species belong to Acidobacteria,Proteobacteria,Chloroflexi and Actinobacteria.There are higher diversity of bacteria than fungi.And there are difference of species diversity between with the fracture and disturbance intensity in differ sample sites.7.There are significantly positive correlationship between AM fungal spore density with the total nitrogen content and soil fungi quantity(P<0.01).And AM fungal spore density is also positively correlated with the plant species richness,the activity of hydrogen peroxide,urease,invertase and phosphatase,the content of organic matter,available nitrogen,available phosphorus content and water content(P<0.05).This study revealed the AM fungal species diversity and distribution characteristics of Shendong coal mining subsidence area of Inner Mongolia,the interaction relationship between AM fungi and bacteria biological diversity with the soil environment factors were be explored depending on the morphological and molecular method,and provide scientific basis for the heath evaluation and protection of biodiversity of mining subsidence area ecosystem.