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Response Of Soil Arbuscular Mycorrhizal Fungi To No-tillage Covering Cultivation And Organic Fertilizer Application

Posted on:2020-11-02Degree:MasterType:Thesis
Country:ChinaCandidate:Z Q WangFull Text:PDF
GTID:2370330578977385Subject:Agriculture
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Arbus mycorrhizal(AM)fungi can form symbiotic relationship with 80%of plants,which plays an important role in improving the nutrient relationship of host plants and changing the diversity of host plants.It provides a theoretical basis for exploring the main environmental factors affecting AM fungi,further revealing the response mechanism of AM fungi to no-tillage mulching and cultivation management measures,and selecting reasonable cultivation and cultivation systems.Taking the soil ecosystem of no-tillage with no-fertilizer(NC),no-tillage with organic fertilizer(NF),no-fertilizer with traditional tillage with no-fertilizer(TC)and organic fertilizer with traditional tillage with organic fertilizer(TF)as research objects,the composition,diversity and difference of soil AM fungi community under no-tillage with cover cultivation were studied by Illumina Miseq high-throughput sequencing technology.Combined with soil environmental factors,redundancy analysis(RDA)was carried out on AM fungal community composition.The results show that:1.Soil nutrients were affected by no-tillage mulching and organic fertilizer application.Total phosphorus(TP)increased with the increase of planting years,while total nitrogen(TN),available phosphorus(AP)and organic carbon(SOC)decreased.Compared with traditional tillage,two-year no-tillage mulching increased the contents of available nitrogen(AN)and total phosphorus(TP),and fertilization measures increased the contents of available potassium(AK)and organic carbon(SOC).2.Compared with traditional tillage and non-fertilization,no-tillage and fertilization are beneficial to the transformation of small aggregates(<0.25mm)to large aggregates(>0.25mm),reducing the content of clay and silt in soil and increasing the composition of sand grains.As far as soil stability is concerned,no-tillage improves the soil water stability coefficient(K)by 48.9%,and increases the stability of soil aggregate structure.3.Compared with the two treatments under the condition of no fertilizer,Ihe fertilization measures all improved the soil respiration intensity,urease,alkaline phosphatase,catalase activity,and compared with the tillage measures,no-tillage increased the soil urease activity.4.Under four different cultivation and management measures,the results of the two-year study found 11 AM fungi genera.The dominant genera are Glomus,Claroideoglomus and Paraglomus.Compared with traditional cultivation,after three years of no-tillage and organic management,no-tillage improved the diversity and richness of AM fungi in soil.The application of organic fertilizer reduced the diversity and richness of AM fungi in cultivated soil and increased the richness index of AM fungi in no-tillage soil.5.Soil environmental factors are one of the important factors that affect the diversity and community composition of AM fungi.Available phosphorus and total phosphorus are positively correlated with the diversity and richness of AM fungi,while pH is negatively correlated with the diversity and richness of AM fungi.
Keywords/Search Tags:arbuscular mycorrhizal fungi, Diversity, High throughput sequencing, No-tillage
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