Isolation,Identification Of Strains From Processing Organic Waste Samples And Strain's Characterization

In order to understand the composition of microorganisms about processing organic waste samples in Japan and to explore the relevant biological characteristics of the strains contained.In this study,strains were isolated from Japanese organic waste samples.Isolates were studied by morphological observation and physical and chemical identification.The growth of observing each strain,at different acidities(pH3.0,pH4.0,pH5.0)different NaCl concentrations(2%NaC1,5%NaCl,8%NaCl),and different culture temperatures(37?,40?,45?,50?).Partial Isolates was identified by nucleic acid identifi-cation.Strain degradation effect of starch,oil and protein,is explored at pH7.0,37?.Results showed that 41 strains were isolated,including strains of 14 Pediococcus acidilactici,15 Bacillus velezensis strains and 13 Pichia farinosa strains.13 Pediococcus acidilactici showed no growth at pH3.0 environment;Pediococcus acidilactici 7,12 and 13 showed good growth at 8%NaCl environment;13 Pediococcus acidilactici showed good growth at 37?,40?,45?,50?.15 Bacillus velezensis?showed no growth at pH4.0 and pH3.0;Bacillus velezensis?A5,A14,A22,A24 and A25 showed growth at 2%NaCl environment;15 Bacillus velezensis showed no growth at 8%NaCl environment and 45?,50?.Pichia farinosa F1,F10 and F13 showed good growth at pH3.0 environment;Pichia farinosa F3 and F4 showed slow growth at 8%NaCl environment;Strain F3,F8,F10,F12,F14,F15 showed slow growth at 50?.Meanwhile,Strain 7,12 13 was Pediococcus acidilactici.strains A5,A14,A20,A24,A25 was Bacillus velezensis;strains F1 and F15 are Pichia farinosa;Both are consistent with biochemical identification results,by nucleic acid identifi-cation.pH 7.0,37? for 24 h,the highest degradation rates of Pediococcus acidilactici 12,Bacillus velezensis A25 and Pichia farinosa F15 were 40.87%,28.91%and 15.72%on starch.The highest degradation rates of Pediococcus acidilactici 7,Bacillus velezensis A25 and Pichia farinosa F15 were 20.28%,56.81%and 53.12%on oil.The highest degradation rates of Pediococcus acidilactici 12,Bacillus velezensis A20 and Pichia farinosa F15 were 53.27%,44.32%and 8.68%in protein.For 48h,the highest degradation rates of Pediococcus acidilactici 7,Bacillus velezensis A24 and Pichia farinosa F1 were 64.58%,76.85%and 45.44%on starch;The highest degradation rates of Pediococcus acidilactici 7,Bacillus velezensis A24 and Pichia farinosa F15 were 84.91%,84.18%and 64.07%on oil.The highest degradation rates of Pediococcus acidilactici 7,Bacillus velezensis A20 and Pichia farinosa F15 strain were 71.38%,70.51%and 70.07%in protein.The results showed Pediococcus acidilactici 7,12,13 strains,Bacillus velezensis A5,A14,A20,A24,A25 strains,Pichia farinosa F1,F15 strains have degradation effects on starch,oil and protein.With the increase of culture time,the degradation rate increased.Pediococcus acidilactici 7 had the best degradation effect on starch,oil and protein.Bacillus velezensis A24 had the highest rate on starch and oil;Bacillus velezensis A20 had the highest degradation rate in protein;Pichia farinosa F1 had the highest degradation rate on starch starch.Pichia farinosa F15 has the highest degradation rate on oil and protein.