Comparative Study On Soil Microbes And Medicinal Components In Rhizosphere Of Rumex Japonicus Houtt Under Different Soil Conditions

In this study,Rumex japonicus Houtt plant and rhizosphere soil(neutral soil and alkaline soil)were used as research objects.Soil microbial community and the content of medicinal ingredients was analyzed by PCR-RFLP technique and high performance liquid chromatography(HPLC).Exploring the relationship between the medicinal components and the microbial community of Rumex japonicus Houtt in neutral soil and alkaline soil,and comparing the content of medicinal components and the microbial community differences in the neutral and alkaline soils.The main conclusions are as follows:1.Changes in microbial community(1)The similarity of bacteria and actinomycetes community in the rhizosphere of Rumex japonicus Houtt in neutral and alkaline soils was higher than that of control soil,and the community was more stable.The similarity of rhizosphere fungi community of Rumex japonicus Houtt in neutral soil was higher than that in control,and the community was more stable,but alkaline soil is the opposite.The rhizosphere microbial community has high diversity and the community distribution is more uniform.(2)The richness and diversity of bacterial community in the rhizosphere of Rumex japonicus Houtt in alkaline soil were higher than that in neutral soil,but the evenness was no significant difference;there was no significant difference in the richness and diversity of rhizosphere fungi community between the two soils,but the evenness in neutral soil was higher than alkaline soil;the richness and diversity of actinomycetes community of Rumex japonicus Houtt in alkaline soil are higher than that in neutral soil,but the evenness is less than neutral soil.Under the two soil conditions,the rhizosphere bacteria,fungi and fungi communities showed a trend of convergence.2.Changes in the content of medicinal components in Rumex japonicus Houtt(1)Under the two soil conditions,emodin was higher in the aboveground part during the sampling period,while chrysophanol,emodin methyl ether and rhein were higher in the underground part;the highest content of emodin in the whole plant,followed by the contents of chrysophanol,emodin methyl ether and rhein were similar;the composition of medicinal components of Rumex japonicus Houtt in the two soils was different in different periods,and the contents of S3 and S4 were the most similar.(2)There was no significant difference in the content of emodin,emodin methyl ether and retinoic acid in the aboveground part of the two soil conditions,and the content of chrysophanol was significantly different.The content of emodin,chrysophanol and emodin methyl ether in the underground part was not significant difference,emodin content was significantly different.There was no significant difference in the content of the four medicinal components in the whole plant of Rumex japonicus Houtt3.Relationship between microbial community and medicinal componentsPearson correlation analysis and multiple stepwise regression analysis showed that the content of emodin in the aboveground part of the Rumex japonicus Houtt was mainly related to the richness of bacteria and actinomycetes.The content of chrysophanol was related to the diversity of fungi and actinomycetes,the content of emodin methyl ether is related to the evenness of bacteria and actinomycetes,and the content of rhein is mainly related to the evenness of fungi and actinomycetes.The content of emodin and rhein in the underground part is related to the richness of bacteria and fungi.The content of chrysophanol and emodin methyl ether is mainly related to the evenness of bacteria and actinomycetes.The content of the four medicinal components in the whole plant of Rumex japonicus Houtt is mainly related to the evenness of bacteria and actinomycetes.4.Correlation between medicinal components of Rumex japonicus Houtt-microbial community-soil physical and chemical properties-enzyme activities(1)In the two soil conditions,there was no significant difference between the p H of the rhizosphere soil and the control soil.The total nitrogen and soil enzyme activities were higher than the control soil,and the organic matter and water content were lower than the control soil.(2)Under neutral soil conditions,the bacteria and actinomycetes had the strongest influence on the content of medicinal components in Rumex japonicus Houtt,followed by total nitrogen and catalase activities.In alkaline soil,p H and catalase activity had the greatest impact on the medicinal components of the Rumex japonicus Houtt,followed by the fungal community.