Correlation Between Rhizosphere Microorganisms From Cinnamomum Migao And Its Medicinal Active Components In Fruits

In order to understand the microbial diversity and differences of Rhizospheric soil of C.migao from different habitats,the relationship between soil physicochemical factors,active ingredients in fruits and the soil bacteria / fungi community was investigated.High throughput sequencing technology was used to investigate the fungal microbiota in rhizosphere soil samples collected in nine C.migao-growing locations of Yunnan,Guizhou and Guangxi provinces.The major active compounds in C.migao fruits and the rhizosphere soil physicochemical factors of the plants were identified.Analysis of correlation of the major active ingredients of C.migao and its physicochemical factors and the diversity of rhizosphere soil bacteria / fungi population,the following research conclusions are obtained:Based on the high-throughput sequencing,the composition of the bacterial community in the rhizosphere soil of C.migao from different habitats was studied and sequenced.319173 valid sequences was obtained and belonged to 29 phyla,63 classes,129 orders,251 families,387 genera and 802 species through the cluster analysis.At the genus level,the dominant group is the unidentified genus Acidobacteria,with a relative abundance of 8.39%.The dominant genera and their relative abundance vary from different regions.The results of the analysis of Alpha diversity showed that Wangmo(WM)> Ceheng(CH)> Leye(LY)> Funing(FN)> Tiane(TE)> Luodian(LD)> Napo(NP)> Zhenfeng(ZF)> Libo(LB);PICRUSt(phylogenetic investigation of communities by reconstruction of unobserved states)was used to determine the metabolic and functional abilities of the observed bacterial communities.High-throughput sequencing analysis of the fungal community composition in the rhizosphere soil of C.migao from testing areas was analyzed.319173 valid sequences was obtained and belonged to 7 phyla,30 classes,89 orders,200 families,440 genera through the cluster analysis.At the genus level,the dominant group is an unclassified fungal genus with relative abundance of 18.76%;the composition of fungal communities in rhizospheric soils of different habitats has obvious differences,The results of the analysis of Alpha diversity showed that Luodian(LD)> Wangmo(WM)> Leye(LY)> Tian'e(TE)> Ceheng(CH)> Libo(LB)>Funing(FN)> Napo(NP)> Zhenfeng(ZF).Based on the FUNGuild software platform analysis,the fungi in the rhizospheric soil of C.migao from nine producing areas contained the following17 ecological functional groups: Ectomycorrhizal,Fungal Parasite-Undefined Saprotroph and Soil Saprotroph etc,Each functional group showed different abundance valus.The research showed that the composition of fungal species in rhizosphere soil of C.migao was abundant and there were some differens in the population structure of soil fungi in the rhizosphere soil and their relative abundance and dominant taxonomic units of different regions.Gas chromatographic(GC)method was used to quantitatively determine the four major active components of C.migao from different areas,The contents of 1,8-cineole,sabinene,limonene and ?-terpineo in the fruit samples from Leye,Wangmo and Tian'e were higher than those of other samples.Redundancy analysis(RDA)showed that the diversity of fungal / bacterial community structure and soil physicochemical factors and the content of four main active compounds(1,8-cineole,Sabinene,limonene,and ?-terpineo)were different degrees of positive correlation.The correlation analysis between the active components from different habitats and the soil rhizosphere fungi taxonomic units showed that,the contents of 1,8-cineole,sabinene,?-terpineo were positively correlated with Agaricus,Fusarium,Phialophora,Cryptococcus,Trichosporon,Archaeorhizomyces;Russula had a positive correlation with limonene to some extent.The bacterial groups such as variibacter,acidibacter,bryobacter and Candidatus?Solibacter are positively correlated with the four medicinal components of C.migao fruits.H16 has a positive correlation with limonene to some extent.The results indicated that the composition of the rhizosphere microbial community affected the synthesis of active compounds of C.migao to a certain extent.