Physiological And Proteomic Analysis Of Puccinellia Tenuiflora Root In Response To H₂O₂

Hydrogen peroxide(H2O2),as one of reactive oxygen species(ROS),plays essential roles in mediating a variety of physiological and developmental processes.The content of H2O2 increases in respond to a variety of biotic and abiotic stimuli,which not only leads to oxidative damage to lipid,protein,and DNA,but can also function as signals to regulate signaling pathways and gene expressions.In this study,we investigated the H2O2 stress response in the root of Puccinellia tenuilfora using a eombined physiological and proteonies approach,focusing on the coverage of the detected protein and differentially expressed proteins.We also analyzed the distribution of isoelectric point(pI)and molecular weight of the differentially expressed proteins.We found that the cell integrity was destroyed,molecules that play osmotic protective roles increased,and defense-related enzymes were affected by H2O2 stress after 6 h in the root of Puccinellia tenuiflora.In addition,we obtained 111 differentially expressed protein spots using two-dimensional electrophoresis(2DE)approach.There are 24 spots differentially expressed(12 spots increased and 12 spots decreased)under 12 mM H2O2,36 spots differentially expressed(20 spots increased and 16 spots decreased)under 18 mM H2O2,and 51 spots differentially expressed(34 spots increased and 17 spots decreased)under 24 mM H2O2.In short,there are a lot of proteins involved in a variety of pathways in the root of Puccinellia tenuiflora in response to the H2O2 stress.These proteins may be involved in the excitation of active 14-3-3 and WD signal transduction pathway,up-regulated proteasome protein expression remit oxidative damage,influence amino acid(Met?Cys?Glu)metabolism,induce cytoskeleton related protein up-regulated expression to enhance cell structure in response to the H2O2 stress.