Quntatitive Proteomics Analysis Of Puccinellia Tenuiflora Root In Response To H₂O₂

Hydrogen peroxide(H2O2)is one of the major components of reactive oxygen species(ROS)and as an important regulatory role in plant development.ROS will be increased after biological and abiotic stress in plants,it is not only leads to proteins,DNA,and lipids damaged by oxidative,but also serves as an important signaling molecule that initiates redox sensors to trigger signal transduction to activate defense responses.In this study,a salt-tolerant Puccinellia tenuiflora was used as material,and iTRAQ-based proteomics techniques were applied to analysis of protein abundance changes in response to stress at different concentrations of H2O2(0 mM,12 mM,24 mM,and 36 mM).In this proteomic analysis,a total of 2,025 abundance-changing protein spots were obtained.Among them,114 kinds of protein abundances were changed significantly,of which 21 kinds of protein abundances were up-regulated,and 93 kinds of protein abundances were down-regulated.The abundance pattern of these proteins suggests that the roots of P.tenuifolia have initiated a variety of H2O2 response strategies,including:(1)P.tenuiflora roots sense and respond to oxidative stress by regulating the abundance of signal transduction related proteins and transmit signals to downstream pathways;(2)The abundance of oxidative stress-associated proteins in P.tenuiflora significantly changes,helping the plants to resist oxidative damage;(3)H2O2 stress breaks the ionic homeostasis in P.tenuiflora roots,which inhibits the expression of transport related proteins.(4)Under the stress of H2O2,protein and fate-related proteins were inhibited in P.tenuiflora roots.