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Physiological And Proteomic Analysis Of Puccinellia Tenuiflora Root In Response To Na2CO3

Posted on:2014-04-15Degree:MasterType:Thesis
Country:ChinaCandidate:Q ZhaoFull Text:PDF
GTID:2370330488499337Subject:Cell biology
Abstract/Summary:PDF Full Text Request
Salinity is one of the major threatens that affect plant production and ecological environment.Puccinellia tenuiflora is a salt-tolerant halophytic species belonging to the Gramineae.It is considered as a good model for studying the plant salt-tolerant mechanism.Plant roots are the primary sites for sensing salt signals.It is important to investigate the salt-responsive mechanism within P.tenuiflora roots.To further understand the Na2CO3-responsive mechanism in roots,P.tenuiflora seedlings were exposed to Ommol/L,150mmol/L,and 200mmol/L Na2CO3 for either 12h or 24h.The biomass and the contents of osmolytes and ions,as well as the antioxidant enzyme activity in P.tenuiflora roots were measured.In addition,the Na2CO3-responsive proteome of P.tenuiflora roots was analyzed using isobaric tags for relative and absolute quantitation(iTRAQ)combined with MS/MS approaches.Based on proteomic analysis,72 Na2CO3-responsive proteins were identified,most of which were involved in signal transduction,stress and defense,transportation,as well as protein synthesis,processing and degradation.Physiological and proteomic results revealed the specific Na2CO3-responsive strategies existed in P.tenuiflora roots.The main strategies include:(1)The growth of roots was slightly suppressed under Na2CO3 stress;(2)Some signaling pathways were triggered in roots to enhance the sense and transduction of stress signaling;(3)Specific antioxidant pathways were activated to scavenging ROS and to reduce the oxidative damages caused by salt stress.(4)Ion homeostasis in roots was disturbed,and the expression of transport related proteins was also suppressed under Na2CO3 stress.(5)Protein synthesis,processing,and turnover in roots was suppressed by Na2CO3 treatment.This provides more important imformation for further underlying the fine-tuned signaling and metabolic networks and regulative mechanisms in P.tenuiflora roots.
Keywords/Search Tags:Puccinellia tenuiflora, roots, Na2CO3 stress, physiology, proteomics
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