POLYPHASIC TAXONOMIC IDENTIFICATION OF Flavihumibacter Stiobioxidans Sp.Nov.AND Lysobacter Tongrenensis Sp.Nov.

The mining explorations always accompany with soil contamination by heavy metals,which will further affect microbial diversity.However,some microorganisms can play an important role on heavy metals removal and transformation,which is useful for the remediation of contaminated soil.In this study,a total of 89 bacterial strains were isolated and identified preliminary using 16S rRNA gene sequencing analysis from soil of an antimony mine and a manganese factory.The predominant genera in the antimony mine soil sample were Pseudomonas,Sphingomonas and Rhizobium.Lysobacter and Thermomonas.were the predominant genera in the manganese factory soil I and soil II,respectively.Two potencial novel strains,YS-17T and YS-37T,were mainly analyzed using polyphasic taxonomic analyses.A Gram-staining positive,strict aerobic,non-motile and rod-shaped bacterial strain,designated YS-17T,was isolated from soil in the Lengshuijiang antimony mine,Hunan province,P.R.China.Comparative 16S rRNA gene sequencing analysis clustered strain YS-17T with the Flavihumibacter strains and was most closely related to Flavihumibacter cheonanensis WS16(97.2%),Flavihumibacter petaseus T41:(96.6%)and Flavihumibacter solisilvae 3-3T(96.5%).The level of DNA-DNA relatedness between strain YS-17T and F.cheonanensis JCM 19322T was 35.5 ± 0.1%(n = 2).The major respiratory quinone of strain YS-17T was menaquinone-7 and the major polar lipids were phosphatidylethanolamine,two unidentified lipids,two unidentified amino lipids and phospholipid.The major fatty acids(? 5%)were iso-C15:0,iso-C15:0 G,unknown ECL 13.565,iso-C17:0 30H,C15:0,C16:1?5c and anteiso-C15:0.The G+C content was 47.8 mol%.Compared to the other Flavihumibacter species,strain YS-17T showed major biophysical and biochemical differences with the abilities to hydrolyze gelatin and assimilation of salicin and L-proline.The results demonstrated that strain YS-17T belongs to the genus Flavihumibacter and represents a novel species for which the name Flavihumibacyer stibioxidans sp.nov.is proposed.The type strain is YS-17T(= CCTCC AB 2016053T=KCTC 52205T).A Gram-staining negative,aerobic,non-motile,rod-shaped bacterial strain,designated YS-37T,was isolated from soil in a manganese factory,P.R.China.Comparative 16S rRNA gene sequencing analysis showed that strain YS-37T clustered with Lysobacter strains and was most closely related to Lysobacter dokdonensis DS-58T(96.0%)and Lysobacter daecheongensis Dae08T(95.8%).The major respiratory quinone of strain YS-37T was ubiquinone-8,and the major polar lipids were diphosphatidylglycerol,phosphatidylethanolamine and phosphatidylglycerol.Its major cellular fatty acids(>5%)were iso-C15:0,iso-C17:1?9c,iso-C16:0,iso-C11:0 3-OH and iso-C11:0.The G+C content of the genomic DNA was 67.1 mol%.Phylogenetic,chemotaxonomic and phenotypic results demonstrated that strain YS-37T belongs to the genus Lysobacter and represents a novel Lysobacter species for which the name Lysobacter tongrenensis sp.nov.is proposed.The type strain is YS-37T(= CCTCC AB 2016052T= KCTC 52206T).The result of this study made contribution to the diversity of the heavy metal resistant bacteria,been significative of the basis research and application adout the microbial resources.I also has participated in during the master graduate student of the function of a new electron transport protein DepH involved with bacterial arsenite oxidation functions related research,and alternate carbon and lipid metabolic pathways underlie arsenite oxidation in Agrobacterium tumefaciens GW4.