The Population Diversity Of Microrganism Under Different Ameliorative Measures In Saline Soil Of Inner Mongolia Areas And The Polyphasic Taxonomic Study Of One Arthrobacter Strain

In this paper,the microbial diversity is briefly introduced,and then two kinds of research methods of soil microbial diversity are described:biological or chemical based methods and molecular biology based methods.Then,high-throughput sequencing technology for microbial diversity was analyzed,and the application of high-throughput sequencing technology in microbial diversity was summarized.The soil microorganisms were treated with no-tillage film series,shallow layer fertilization series and straw-saving irrigation series from Inner Mongolia saline soil,respectively.The soil microorganisms were analysed by culturable microbial analysis or high-throughput sequencing.The culturable microbial experiment results showed that no-tillage and shallow tillage treatments could effectively increase the number and type of microorganisms in the saline soil,and 10%of conventional irrigation could be saved after the laying of straw.The results of high-throughput sequencing showed that the diversity of microbial diversity was higher in no-tillage film mulching treatment in saline-alkali soil,and the microbial diversity was lower in shallow layer.In addition,a bacterial strain NJ-Z5 ^T isolated from soil near the Antarctic Great Wall Station through the laboratory was systematically classified in this paper.The 16S rRNA gene sequence similarities of strain NJ-Z5^T to the most closely related specie Arthrobacter sulfonivorans JCM13520^T is 98.07%.The DNA-DNA relatedness values of strain NJ-Z5^T with Arthrobacter sulfonivorans JCM13520^T was 47.23%,which were below the recommended criterion of 70%for species delineation.Strain NJ-Z5^T was Gram-positive,aerobic,non-mobility and rod-shaped,its colonies were ivory-white,it was found to grow at NaCl concentrations of 0-1%w/v,temperatures of 4-28??optimum 25??and pH6.0-8.0?optimum pH 7.0?.Positive for nitrate reduction,gelatinase,catalase,hydrolyze arginine,esculin,and urea.Negative for oxidase,H₂S production,starch hydrolysis,Voges�Proskauer test and MR test.The predominant menaquinone was MK-9?H₂?,MK-9,MK-10?H₂?and MK-8?H₂?,the major fatty acids were anteiso-C_15:0?iso-C_16:0?anteiso-C_17:0?C_16:1 w7c/_16:1?6c and iso-C_15:0,the polar lipid acids were DPG,PG,GL and UPL.Phylogenetic,chemotaxonomic and phenotypic analyses indicated that strain NJ-Z5^T represents a novel species within the genus Arthrobacte,for which the name Arthrobacter albocanus sp.nov.is proposed.