Expression Pattern Analyses Of Receptor-like Kinases In The Ovule Of Arabidopsis Thaliana

Arabidopsis thaliana receptor-like kinases(RLKs)form a monophyletic gene family with at least 620 members,and play important roles in many processes,such as growth,development,defence response,and innate immunity.A typical RLK contains an extracellular domain,a transmembrane domain,and an intracellular kinase domain;About 30%of the Arabidopsis RLKs have not the extracellular domain,with either the transmembrane domain and the intracellular kinase domain,or only intracellular kinase domains.Previous studies already showed that RLKs play vital functions in reproductive development and double fertilization.However,further researches will be needed to identify more RLKs involved in these processes and to elucidate the detailed mechanisms.In this study,promoter sequences of RLKs(except LRR-RLKs)were cloned by the Gateway in vitro DNA recombination technology and transferred into a destination vector,pBIB-Basta-GUS-GWR.All of the resulted expression constructs were introduced into Agrobacterium and then transformed into wildtype Arabidopsis by floral dip.Corresponding transgenic plants of all 376 RLKs were obtained and observed after GUS staining.A total 68 RLKs show expression in mature ovule,and 23 of them specifically express in the embryo sac.These genes probably regulate the development of ovules or female gametophyte and female gamete,and the recognition between male and female gametophytes.