Analysis Of Expression Patterns Cry1, Cry2, And COP1 Promoters In Arabidopsis

Arabidopsis Cryptochromes(CRY), including CRY1 and CRY2, are blue light receptors whose signaling pathway is critical for the regulation of the developmental growth. In its signaling pathway, CRY physically interacts with COP1, a RING-finger containing E3 ligase that acts as a negative regulator in photomorphogenic development. However, the expression pattern of CRY1, CRY2 and COP1 gene under different light conditions and in different organs are largely unknown. In this study, the expression of CRY1::GUS, CRY2::GUS and COP1::GUS reporter genes are examined in transgenic Arabidopsis. According to GUS histological staining results, the expression patterns of three key genes acting in the pathway of light signal transduction pathway, namely CRY1, CRY2 and COP1, are not regulated by light conditions in wild type Arabidopsis thaliana. The GUS staining results also showed that CRY1, CRY2 and COP1 are extensivly expressed in all organs of grown plants. By analysing the expression pattern of the same gene in both wild type and mutant background, we concluded that there are no obvious transcriptional interactions between CRY1 and COP1.