| BackgroundIn recent years,as the prevalence of hypertension increases year by year,the population of hypertensive renal damage is also increasing.Hypertensive renal damage is one of the common causes of end-stage renal disease(ESRD),and early prevention and treatment can effectively reduce the incidence of ESRD.In the early stage of hypertension,renal damage is mainly caused by renal tubule damage,and glomeruli can be involved in late stage,which can cause ischemic sclerosis,affect renal perfusion and reduce glomerular filtration rate.Therefore,early intervention is the key to delay renal function deterioration in patients with hypertensive renal damage.According to the reports,Xia Yu Xue Tang can play the role of renal protection from multiple angles,but little has been reported about its protective effect on renal tubules.In the early stage of hypertensive renal damage,the increase of nocturia,urine osmotic pressure,urine microalbuminuria,and urine N-acetyl-beta-D-aminoglucosidase(NAG enzyme)rise,water channel protein 2(AQP2)is essential for the maintenance of normal water metabolism.Our previous work has proved that Xia Xue Decoction has the effect of reducing the number of nocturnal urine of patients with hypertensive renal damage and reducing the effect of urinary NAG enzyme and 24 hours urine microalbumin.This study is to verify the protective effect of Xia Hui Xue Decoction on renal damage in spontaneously hypertensive rats and explore the possible mechanism of renal protection by animal experiments.The bed provides a new way of treating traditional Chinese medicine for hypertensive renal damage.ObjectiveTo observe the possible renal protective mechanism of blood pressure,renal function,urine NAG enzyme,urinary AQP2,renal AQP2 expression and renal pathological changes in rats treated with Xiayuxue Tang in spontaneously hypertensive renal impairment rats.Explore new treatment options for clinical treatment of hypertensive renal damage.MethodThe 14 weeks old male Wistay-Kyoto rats(WKY)and spontaneously hypertensive rats(SHR)were randomly divided into 6 groups at the age of 15 weeks,and 8 rats in each group were randomly divided into normal group(saline),model group(saline),Chinese medicine.Group(given lower blood Stasis Decoction),western medicine group(given to the side of the party),low dose group(to give western medicine + low dose of Chinese Medicine),high dose group(to give western medicine + high dose of Chinese Medicine).The rats were fed with 1 cages per 4,constant temperature and humidity,and fed with standard rats for 8 weeks.During the experiment,the general condition of rats was recorded and blood pressure was measured(0 weeks before administration,fourth weeks after administration,eighth weeks after administration),renal function,urine NAG enzyme,urine AQP2(respectively before and after administration).After 8 weeks of treatment,the kidney tissues were left in each group.Paraffin section was stained by HE to observe the pathological changes of kidney tissue and the immune group The expression of AQP2 in the kidney was observed.Method of blood collection:0 weeks after administration,blood was taken from orbit,and the rats were anesthetized for 8 weeks and blood was taken from abdominal aorta.All data were analyzed by SPSS20.0 statistical software.Result1,General condition of ratsCompared with the normal group,the spontaneously hypertensive rats had strong activity,good fighting,quiet,easy to be frightened,poor adaptability and strong sensitivity to sound stimulation.After administration,the rats in the Chinese medicine group,the western medicine group,the high dose group and the low dose group were generally in good condition,the hair and lustre were smooth,the diet was as usual,the diet was as usual,and gradually adapted the feeding environment.2,Blood pressure in ratsCompared with the model group,the blood pressure of the eighth week western medicine group and the low dose group was significantly lower than that of the previous one,with significant statistical significance(P<0.01).The blood pressure of the high dose group was lower than that of the former(P<0.05).The blood pressure of the Chinese medicine group was lower than the model group(P>0.05),and the low dose group was better than the low dose group.In western medicine group,there was no statistical significance(P>0.05).3,Renal function in rats(blood creatinine,urea nitrogen,blood uric acid)In terms of serum creatinine,the serum creatinine of the Chinese medicine group,the western medicine group,the high dose group and the low dose group decreased more than before the treatment,but there was no statistical significance(P>0.05)compared with the model group(P>0.05).Compared with the model group,the high dose group and the low dose group were significantly lower than the model group.There was significant statistical significance.P<0.01),the decrease of the high dose group was better than the low dose group,the urea nitrogen level of the traditional Chinese medicine group and the western medicine group decreased,but there was no statistical significance(P>0.05).The blood uric acid in the blood uric acid group and the western medicine group had a lower blood uric acid than before the treatment,and there was no statistical significance compared with the model group(P>0.0 5).4,Urine analysis of rats(urine NAG enzyme,urine AQP2)In terms of urinary NAG enzyme,compared with the model group,the urinary NAG enzyme of the Chinese medicine group,the western medicine group,the high-dose group,and the low-dose group had no significant change in the 8th week after administration(P>0.05);urine AQP2 On the other hand,compared with the model group,the urinary AQP2 in the Chinese medicine group,western medicine group,high-dose group and low-dose group did not change significantly compared with before,and there was no statistical significance(P>0.05).5,Pathological analysis of kidney in ratsCompared with the normal group,the glomerular mesangial matrix increased,the glomerular sac narrowed,the renal proximal tubule stenosis,and some renal arteriosclerosis in the model group had significant differences.Compared with the model group,there was no obvious turbid edema in the renal tubular epithelial cells in the western medicine group.The renal tubular lesions in some rats were significantly less than those in the model group.There were no obvious pathological changes in the kidney of the other rats.The low dose group:the renal tubular lesions were significantly lighter than the model group,glomerular lesion and renal arteriopathy.There was no obvious difference in the model group.There was no obvious lesion in the renal tubule in the high dose group.The glomerular segmental sclerosis and fibrous hyperplasia in some rats were not found in the other rats.The renal tubules in the other rats had no obvious pathological changes,and there was a significant reduction in the model group.The atrophy of glomeruli and glomerulosclerosis in the model group was less than that in the model group,but there was no statistical difference.6,Immunohistochemical results of AQP2 in rat renal tissueThe positive staining of AQP2 was located in the membrane and cytoplasm of the main cells of the renal collecting ducts.The expression level of AQP2 in the kidneys of the model group was significantly higher than that of the normal group.Compared with the model group,the expression of AQP2 in the kidneys of the low-,high-dose and Chinese medicine groups was significantly reduced.The expression of AQP2 in the kidneys of the group decreased,but there was no statistical difference.Conclusion pressure1,Xiayuxue Tang can improve the general condition of hypertensive renal damage rats.2,Xiayuxue Tang can reduce the blood pressure of hypertensive kidney damage rats.3,Xiayuxue Tang can reduce the level of serum creatinine,blood uric acid and urea nitrogen in rats with hypertensive kidney injury.4,Xiayuxue Tang can alleviate renal pathological damage in rats with hypertension and kidney damage.5,Xiayuxue Tang can reduce the expression of AQP2 in renal tissue of rats with hypertension and renal damage to protect the kidneys. |
PDF Full Text Request