| Essential hypertension is a high incidence of cardiovascular disease. In recent years, the incidence of young people tend to. Chinese medicine will be attributed to high blood pressure for dizziness,liver and other Chinese medicine name, emphasizing the occurrence and development of hypertension and Tibetan theory of liver function disorders are closely related.For the young people with high blood pressure, TCM syndrome is mostly anger. Xiateng compound is based on Prunella vulgaris,Uncaria Steven Medicine,supplemented by Cassia,Salvia, Achyranthes bidentata, for the anger of hyperthyroidism etiology and pathogenesis, all drugs combined, played a total of Qinghu anger, Which has good clinical efficacy in lowering blood pressure, improving patient symptoms, reducing or delaying complications, and so on.However, the mechanism of lowering blood pressure is not clearObjective:Guided by the idea of "macroscopic evaluation of drug efficacy and microscopic exploration" and Based on the quantitative model of disease and syndromes,this study was based on the animal model of stagnation of anastomosing hypertensive rats (14 to 18 weeks old), and from the aspects of disease and syndrome To evaluate the effect of Xiateng compound on the changes of heart and thoracic aorta and the effect of vasoactive substances in rats with anger hyperactivity hypertension. To explore the possible mechanism of Xiateng compound for reducing blood pressureMothods:1. In this study, based on the previous research results, according to the high blood pressure hyperactivity syndrome macro-scale scale of spontaneously hypertensive rats to identify the syndrome, the initial screening to meet the anger of the SHR rats, and then by the standard of clinical syndrome equivalent to the quantification of the main symptoms and secondary macroeconomic indicators,the establishment of a stable type of anger hyperactivity hypertension syndrome model.2. 45 rabbits with 14 weeks of age were randomly divided into 5 groups: model group,nifedipine sustained release group,high,medium and low dose group,9 WKY rats,As a normal control group. Continuous administration for r 4 weeks, weekly blood pressure measurement,rotation tolerance test,open field experiment,easy to provoke degree score,secondary disease score. From the aspect of disease and syndromes, the efficacy of antihypertensive efficacy was evaluated.3 .After 4 weeks of administration, the material was taken. Left ventricular mass (LMI)was calculated and the left ventricular mass index (LVMI) was calculated. The heart and thoracic aorta were stained with HE and the myocardium and thoracic aorta were observed under light microscope Pathological changes.4.ELISA method detection of rat serum angiotensin ? (ANG ?), norepinephrine (NE),endothelin (ET), calcitonin gene related peptide (CGRP) thromboxane B2 (TXB2), 6- keto prostaglandin F1 (6-Keto-PGF1 alpha) content.Results:1. Anger hyperactivity syndrome model group compared with normal group, systolic blood pressure, rotation tolerance time shortened, in case the increase in the number of irritability score increased, secondary symptoms increased (P<0.05).2.Before and after administration for 4 weeks, the high, middle and low dose groups of Xiateng compound could reduce or inhibit the blood pressure in different degrees, and the highest dose of compound was the best (P<0.05). Xiateng compound high, middle and low dose groups of rats with hyperactivity of liver fire type rotation tolerance time, the number of crossing line, irritability score, secondary symptom score has been improved to some extent.3.After 4 weeks of administration, the left ventricular tissue morphology was observed.Compared with the normal group, the cardiomyocytes in the model group were significantly increased and the disorder was observed. The gap between the cells was widened and the inflammatory cells were infiltrated around the tissue. The myocardial fibers of the high,medium and low dose groups were gradually neat, the infiltration of inflammatory cells was gradually reduced, and the pathological damage of myocardium was improved to some extent.The effect of the high dose group was the best. The morphology of thoracic aorta was observed. Compared with the normal group, the intima of the thoracic aorta was thickened,the proliferation of vascular smooth muscle cells, the arrhythmia of vascular endothelial cells were disturbed, and the reconstruction of vascular wall structure was observed. The high,medium and low dose groups of Xiateng compound can inhibit the reconstruction of vascular wall structure.4. After 4 weeks of administration, the contents of ANG? NE, ET and TXB2 in serum were decreased compared with the model group, and the content of CGRP in serum was increased (P <0.05).Conclusion:1 Xiateng compound can effectively reduce or inhibit the anger of hyperthyroidism SHR systolic blood pressure to further increase, and can significantly improve the hypertensive rats with anger manifestations of macro-symptoms, can make SHR weight gain significantly,which reflects the traditional Chinese medicine compound On the overall role of the body to adjust the advantages.2. Xiateng compound for heart tissue can effectively reduce the left ventricular weight index, reduce the SHR cardiac hypertrophy, with the protection of myocardial cell damage,improve the role of myocardial fiber remodeling; for vascular tissue to protect the vascular endothelium.3.Xiateng compound can reduce the levels of ANG, NE, ET and TXB2 in serum, and increase the content of CGRP in serum, which may be the mechanism of inhibiting blood pressure and protecting the heart and blood vessels. |
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