Based On NF-kB Signaling Pathway, The Effects Of Xiaoqinglong Decoction And Yupingfeng Powder On The Expression Of MUC5AC And MUC5B In Rats With Allergic Rhinitis Were Investigated

Purpose:To observe the effect of the Xiaoqinglong Decoction and Yupingfeng Power on the expression of MUC5 B and MUC5 AC in nasal mucosa of allergic rhinitis rats by NF-kappa B signaling pathway,and reveal the mechanism of the Xiaoqinglong Decoction and Yupingfeng Power treating AR.Material and method:48 healthy male wistar rats were divided into 6 groups by randomized bocks design: normal group,AR model group,Forskolin(c AMP activator)group,H89(PKA inhibitor)group,PDTC(NF-kappa B inhibitor),and the Xiaoqinglong Decoction and Yupingfeng Power group,8 rats in each group.Except for the normal control group,other groups use ovalbumin and aluminum hydroxide as sensitization liquid,intraperitoneal injection unites the local attack made a rat model of AR.All groups were given drug intervention for 2 weeks,then we observe rat behavior index score(nose scratching,sneezing,runny nose)improvement and the expression of MUC5 B and MUC5 AC,the results were statistically analyzed.Results: 1.Comparison of behavioral index score of rats after treatment: Compared with the blank control group,the scores of behavioral index in model group were significantly higher(P<0.01);compared with the model group,the behavior index score of Forskolin intervention group,PDTC intervention group and Xiaoqinglong Decoction and Yupingfeng Power group were significantly improved(P<0.01,P<0.01,P<0.01);Compared with the model group,the nasal scratching frequency and increased the frequency of sneezing in H89 intervention group were increased(P<0.05,P<0.01).2.MUC5 AC Western blotting results: Compared with the blank control group,the expression of MUC5 AC in model group was significantly higher(P<0.01);compared with model control group,the expression of MUC5 AC in Forskolin intervention group,PDTC intervention group and Xiaoqinglong Decoction and Yupingfeng Power group were significantly lower(P<0.01,P<0.01,P<0.01); compared with model control group,MUC5 AC expression in H89 intervention group was significantly increased(P<0.01).3.MUC5 B Western blotting results: Compared with the blank control group,the expression of MUC5 B in model group was significantly higher(P<0.01);compared with model control group,the expression of MUC5 B in Forskolin intervention group,PDTC intervention group and Xiaoqinglong Decoction and Yupingfeng Power group were significantly lower(P<0.01,P<0.01,P<0.01);compared with model control group,MUC5 B expression in H89 intervention group was significantly increased(P<0.01).Conclusion: 1.Xiaoqinglong Decoction and Yupingfeng Power group can significantly improve the behavioral index score of allergic rhinitis rats,alleviate its symptoms and have good effect.2.The expression of MUC5 AC and MUC5 B protein in rats with allergic rhinitis can be decreased by Xiaoqinglong Decoction and Yupingfeng Power group.3.The mechanism of Xiaoqinglong Decoction and Yupingfeng Power group in treating allergic rhinitis may be related to inhibition of NF-kappa B signaling pathway,excitatory c AMP-PKA signaling pathway,and down regulation of MUC5 AC and MUC5 B expression...