Based On "heart And Small Intestine Phase", The Effect Of Yangxin Tongmai Recipe On Intestinal Flora Of Rats With Coronary Heart Disease And Blood Stasis Syndrome Was Discussed

ObjectiveHigh fat diet and intraperitoneal injection of vitamin D₃ and isoproterenol?ISO?were used to prepare the rat model of coronary heart disease with blood stasis syndrome.To explorethe relationship between coronary heart disease with blood stasis syndrome and intestinal microbes and the effect of Yangxin Tongmai decoction on intestinal microbes in rats of coronary heart disease with blood stasis syndrome on the base of“the Heart and the Small Intestine Share a Paired Relationship”of the visceral outward manifestation theory.MethodsThe rats were divided into normal group and model group.The rats in model group were treated with high fat diet and intraperitoneal injection of vitamin D₃ and ISO to prepare the rat model of coronary heart disease with blood stasis syndrome.After the model was completed,the model rats were divided into four groups:coronary heart disease with blood stasis syndrome group,natural recovery group,atorvastatin calcium group,and Yangxin Tongmai decoction group.Escherichia coli,bifidobacterium,lactobacillus,and fungi in the intestinal contents of the rat small intestine?5 cm above the ileocecal valve?were cultured after the intervention of Yangxin Tongmai decoction for two weeks.The relationship between blood stasis syndrome and intestinal microflora in rats with coronary heart disease and the effect of Yangxin Tongmai decoction on intestinal microflora were analyzed.Results1.After the rat model was completed,the blood lipid,blood rheology and other indicators of model rats were significantly higher than those of the healthy control group,and the difference was statistically significant?p<0.05orp<0.01?.The ECG results showed that the J point of lead II were lifted or moved down,and the T wave was low or inverted.The results of pathological examination showed that the rat model of chronic coronary heart disease with blood stasis syndrome was successfully reproduced.2.The results of intestinal flora culture showed that,compared with the healthy control group,the E.coli and fungi of each group were increased,and the difference was statistically significant?p<0.05orp<0.01?.Lactobacillus and Bifidobacterium were significantly reduced,especially in rats with coronary heart disease blood stasis more obvious,the difference was statistically significant?p<0.01?.3.The application of Yangxin Tongmai Recipe can reduce the indexes of TCHO,TG and LDL-C,and increase the value of HDL-C index,compared with the rats in coronary heart disease,blood stasis syndrome group and natural recovery group.The difference was statistically significant?p<0.05 or p<0.01?.The TG index of Yangxin Tongmai group is lowers than atorvastatin calcium group(?p<0.05?.The application of Yangxin Tongmai formula can reduce the whole blood viscosity low shear and thewhole blood viscosityhigh shear,erythrocyte aggregation index,hematocrit value index,compared with the blood stasis syndrome of coronary heart disease group and natural recovery group values,the difference was statistically significant?p<0.05 or p<0.01?.On the reduction of blood viscosity low shear and blood viscosity high shear,erythrocyte aggregation index,Yangxin Tongmai group was superior to atorvastatin calcium group,and the difference was statistically significant?p<0.05?.4.After the application of Yangxin Tongmai decoction,Yang Xin Tongmai decoction can remarkably reduce the number of E.coli,compared with coronary heart disease,blood stasis group,natural recovery group and atorvastatin calcium group,the difference was statistically significant?p<0.01?.Compared with coronary heart disease and blood stasis group,natural recovery group,the number of fungi inrats of Yangxin Tongmai group decreased?p<0.05orp<0.01?.The number of Lactobacillus and Bifidobacterium in Yangxin Tongmai formula group increased and recovered quicklycompared with the coronary heart disease blood stasis syndrome group and atorvastatin calcium group.The difference was statistically significant?p<0.05 or p<0.01?.Conclusion1.There issevere inflammatory responsein rats of coronary heart disease blood stasis syndrome.The number of lactic acid bacteria and bifidobacteria in the blood stasis rats with coronary heart disease decreased obviously,the number of Escherichia coli and fungi increased obviously,and the blood stasis syndrome of coronary heart disease may interact with the imbalance of intestinal flora.2.Yangxin Tongmai decoction can effectively improve blood lipid and blood flow rheology index in rats with coronary heart disease and blood stasis syndrome.Yangxin Tongmai decoctionis beneficial to the intestinal flora of rats with coronary heart disease blood stasis syndrome,including the recovery of Lactobacillus and bifidobacteria.It is helpful to improve the pathological state of intestinal microflora in rats with chronic coronary heart disease and blood stasis syndrome,and to maintain the diversity of intestinal flora in rats,which may be one of themechanisms of Yangxin Tongmai decoction in treating coronary heart disease.3.Through the multi-target,multi-channel intervention,Chinese medicine can adjust the composition of intestinal microflora to achieve balanced intestinal microecology,improve intestinal micro-ecological environment,so as to improve the pathological status of coronary heart disease blood stasis rats and treat the coronary heart disease.