Effects Of Celecoxib On The Production Of Traumatic Ossifying Myositis And The Expression Of BMP-4/Smad Signaling Pathway In Rats

Objective: To study the effect of celecoxib for tendon cut induced rat model of traumatic myositis ossificans and BMP- 4 / Smad signaling pathways of expression, the effect of celecoxib prevention rats molecular biology mechanism of traumatic myositis ossificans.Methods: 109 rats, to cut off the Achilles tendon induced rat model of traumatic myositis ossificans, were randomly divided into blank control group, model group and drug group, respectively give corresponding intervention. Observed in rats and its Achilles tendon in general situation, imaging examination and pathological examination to determine efficacy. Using immunofluorescence test and RT-PCR to test expression of BMP- 4 / Smad gene signal transduction pathways.Results: the general view: The blank control group had no significant change,model group and drug group with time gradually extend the week of the Achilles tendon diameter enlargement, elastic reduction, harden, and lateral local temperature gradually increased, compared the three way all remaining difference between groups(p < 0.05).X-ray: the blank control group without calcification or ossification signs, model group has 100% ossification int the 6th week, drug group has100% ossification in the 10 th week, model group Achilles tendon ossification development rate is always higher than that of drug group, and a significant difference was found in 4, 6 weeks(p < 0.05).Pathology examination: the model group, medication group ossification trend gradually increase over time, the model group the Achilles tendon 4 weeks into cartilage, cartilage or bone cells began to appear, in 6 weeks has 100% ossification signs; Drug group in 6weeks beginning see osteogenesis phenomenon, all 10 weeks in ossification.Immunohistochemical fluorescence detection: blank control group except Smad5 are expressing the rest; Model group, medication group, Smad1 BMP- 4, 5, 8 are all expressed,and showed a trend of rising and expression level was higher than the blankcontrol group; Compared with drug group, model group of BMP-4 to 6, 8 weeks were significantly increased(p < 0.05), entire Smad1, 5 higher than drug group, but Smad5 there was no significant difference in the 10 weeks and drug group(p < 0.05), Smad8 in week 4 positive rate was higher than drug group(p < 0.05). RT-PCR detection: the whole experiment process and BMP-4 Smad8 expressed in three in the experimental group were increased, comparison between groups, expressing quantity model > drug group > blank control group(p < 0.05). Smad1, Smad5 relatively large variations in the process of the experiment, a complementary role relationship; Drug group, Smad1 and Smad5 4 to 10 weeks in exactly the same as that of model group express the overall trend, but the expression amount was lower than those of model group(p < 0.05).Conclusion: 1. Celecoxib has certain effect. for early prevention and cure of traumatic myositis ossificans. 2. Traumatic myositis ossificans formation mechanism by BMP-4/Smad1, 5, 8 signal pathway induced into differentiation of bone cells, resulting in partial ectopic ossification of the body. 3. BMP-4 induced heterotopic ossification process, Smad1, 5, 8, mainly for synergy. 4. Celecoxib early prevention and control of the main mechanism is traumatic myositis ossificans to inhibit the expression of BMP-4, thus inhibiting Smad1, Smad 5, Smad8 induce extracellular factor into the nucleus,prevents the directional differentiation of osteoblast.