A New Tactics For Label-free Recognition Of β-trophin Via Electrochemiluminescent Signaling On Immuno-interface With AuNPs Supporting

In this paper,a new strategy to prepare a label-free electrochemiluminescent（ECL）immunosensor forβ-trophin detection was reported,with good specificity,reproducibility and acceptable stability.An aquogel polymer from the hydrolysis of（3-aminopropyl）trimethoxysilane（APTMS）acted as the linker to catch the Au nanoparticles（AuNPs）onto the ITO substrate by a novel one-step method.The AuNPs play an important role in enhancing the ECL and immobilizing theβ-trophin antibody.This immunosensor can testβ-trophin by using luminol as ECL probe.The ECL intensity on resultant sensor,after the direct immuno-interaction,was proportional to the concentration ofβ-trophin and owned a linear response range from 3μg mL^-1 to 13.6μg m L^-1,with a low detection limit of 1.19μg mL^-1.The proposedβ-trophin sensor had great potential in the determination ofβ-trophin in human serums,which showed important value for the medical research of cardiovascular disease and diabetes.