A New Tactics For Label-free Recognition Of ?-trophin Via Electrochemiluminescent Signaling On Immuno-interface With AuNPs Supporting

In this paper,a new strategy to prepare a label-free electrochemiluminescent?ECL?immunosensor for?-trophin detection was reported,with good specificity,reproducibility and acceptable stability.An aquogel polymer from the hydrolysis of?3-aminopropyl?trimethoxysilane?APTMS?acted as the linker to catch the Au nanoparticles?AuNPs?onto the ITO substrate by a novel one-step method.The AuNPs play an important role in enhancing the ECL and immobilizing the?-trophin antibody.This immunosensor can test?-trophin by using luminol as ECL probe.The ECL intensity on resultant sensor,after the direct immuno-interaction,was proportional to the concentration of?-trophin and owned a linear response range from 3?g mL^-1 to 13.6?g m L^-1,with a low detection limit of 1.19?g mL^-1.The proposed?-trophin sensor had great potential in the determination of?-trophin in human serums,which showed important value for the medical research of cardiovascular disease and diabetes.