The Research Of Uric Acid,Alanine Aminotransferase And Choline Biosensors Based On Electrochemiluminescent Of Luminol

This paper studied and fabricated ECL-Biosensor based on the signal output of luminol's ECL in enzymatic reaction. Because of H2O2 produced by enzymatic reaction can increase the ECL of luminol with this can it be used to fabricate different enzyme biosensors. They can detect the lower concentration of samples due to the highly sensitivity of ECL and highly selectivity of enzyme.The first part of this paper studied the enhancement effect of Potassium Ferricyanide on the ECL intensity of luminol, the results revealed that Potassium Ferricyanide can enhance the oxidation of luminol, which can increase the ECL intensity; it can used to detect the concentration of uric acid in porphyra with satisfactory accuracy and reproducibility.The second part studied the fabrication of ECL-uric acid biosensor, and discussed the enhancement effect of Potassium Ferricyanide on uricase reaction. An ECL Biosensor based on uricase and polypyrrole (PPy) film on platinum electrode has been studied, and it can increase the ECL intensity of luminol. The linear range of uric acid was 7.5×10^-11mol/L-8.3×10^-6mol/L. This ECL-biosensor showed an excellent character for measuring uric acid, and enzymatic membrane is very stable.The third part studied the fabrication of ECL-alanine aminotransferase (ALT) biosensor; ALT can catalyze the reaction of alanine andα-ketoglutarate which can produce pyruvic acid and glutamic acid, pyruvic acid can react with Pyruvate oxidase to produce H₂O₂ which could increase the ECL intensity of luminol. The linear range was from 0.00475 to350 U /L. It was used to determine the concentration of ALT in serum.The last part studied the fabrication of ECL-choline, it also based on H₂O₂ produced by the catalysis of choline oxidase to choline. The liner range was from 2×10^-7mol/L to 4×10^-3mol/L, and was used to determine the concentration of choline in serum.