Evaluation Of Respiratory Metabolism In Macrophages Of Mice Based On Electrochemiluminescent Imaging At Microchips

Along with people's demond on the food safety, environmental protection and clinical diagnosis, developing new analytical apparatuses and methods with high sensitivity, wide versatility and user-friendliness was urgently required. Electrochemiluminescence (ECL) has shown great potential in solving this problem. ECL, where CL is triggered and controlled by electrochemical reaction, possesses its intrinsic electro-stimulated sensitivity, spatiotemporal regulability, wide dynamic rang and good controllability. ECL has become a commercialized platform.Porphyrin, widely used in the field of ECL, are excellent organic electrolum inescent materials. Generally, porphyrins can emit satuared red via energy transfer from hosts wether they are doped or incorporated into polymric materials.Recently, ECL imaging has been emerged as a novel technique in the field of ECL. Differ from intensity signals from traditional ECL, visualization of ECL can offer clear images, which is beneficial for high-throughput analysis and visible assays. Based on the ECL imaging technique, novel methods for biomolecules detection have been reported in this thesis. This postgraduate dissertation contains the following three parts about the ECLimaging.Mechanism of Electrochemiluminescent Visualization Induced by Electroactive Zinc Porphyrin Microchip in Aqueous MediaIn this work, Distinct from the ECL inability of numerous organic species in aqueous solutions, a strong and stable red irradiation at 634 nm could be stimulated electrochemically on zinc (?)/meso-tetra(4-carboxyphenyl) porphine (ZnTCPP)/tetraoctylammonium bromide (TOAB) in the physiological condition. In terms of in situ electron paramagnetic resonance and ECL spectroscopies, the nature of ECL was thoroughly investigated, being exactly the chemiluminescence from singlet oxygen produced during the successive electro-reduction of ZnTCPP. Meanwhile, the excellent film-making capacity of amphiphilic TOAB as a potent ion barrier granted the luminophores a micro-order and patternable electrode modification.ECL Imaging Based on TOAB/ZnTCPP Microchip for the Evaluation of Respiratory MetabolismThis work proposed a TOAB/ZnTCPP Microchip used in visual detection by powerful ECL property and enhancement from peroxide. Peroxide produced by cellular respiration and photosynthesis in plants, significant in research on cellular aerobic respiration. The pixel was proportional to the concentration of peroxide, thus an ultrasensitive assay was constructed with visualization and lower detection limit.Porphyrin-Based Metal-Organic Framework for Electrochemiluminecnt BiosensorIn this work, silanized ZnTCPP which has powerful ECL intensity and compatibility as the monomer of MOF possess advantages of regular conformation, cecellent conduction, high efficiency ECL. The porosity of MOFs enhance the ability of gas stotage and improve efficiency ECL. Thus, microchip based on MOF provide a more sensitive and efficient imaging techique.