Study On Technology And Applications Of Singlet Oxygen Luminescence Imaging In Blood Vessels

Singlet oxygen(1O2)as the major cytotoxicity species in type ? photodynamic therapy(PDT),has a phosphorescence at 1270 nm.As the development of PDT,quantification and modulation of 1O2 generation still is a critical technique in PDT-1O2 dosimetry research.In this paper,a novel 1O2 luminescence imaging system was developed by our group.Then,the photosensitized 1O2 images were obtained successfully under solution environment and from a nude mice dorsal skin window chamber in vivo.Moreover,the preliminary dose-effect relationship between the 1O2 and PDT effect on treated blood vessels was investigated.These results were very helpful to reveal the mechanism in vascular damage for vascular targeted photodynamic therapy(V-PDT).The main research contents of this dissertation are introduced as follows:Firstly,a rapid,high-resolution and reliable 1O2 imaging system was developed by our group.Moreover,the photosensitized 1O2 luminescence images from Rose bengal(RB)solution was obtained successfully with a resolution of 30 ?m.Secondly,1O2 luminescence images photosensitized by RB photosensitizer from a nude mice dorsal skin window chamber were implemented successfully in vivo.The 1O2 luminescence images under different irradiation power densities and photosensitizer concentration were obtained,respectively.Finally,1O2 luminescence signal was confirmed again by a hypoxic condition.Results showed that our 1O2 luminescence imaging system could be employed to imaging 1O2 luminescence in vivo.Thirdly,different parameters in V-PDT were implemented to induced different targeted blood vessels V-PDT damage in blood function.The vasoconstriction was quantified by a stereomicroscope to measured the diameter of targeted blood vessels before and after V-PDT.As a result,the dose-effect relationship between the 102 luminescence intensity and V-PDT damage of targeted blood vessels was shown as a positive correlation..Finally,a laser speckle blood flow imaging system was used to monitor the targeted blood vessels V-PDT damage by quantitative class blood flow change after V-PDT.Result shows that there is positive relationship between the 1O2 luminescence intensity and blood flow change.Futhermore,the dynamic V-PDT resoponse of different type blood vessels was monitored by the laser spekle imaging system.The result shows that the artery has a more sensitive V-PDT response than that on the vein vessels.In conclusion,the preliminary result of 1O2 based dosimetry in V-PDT experiment is very valuable for the clinical V-PDT research.