The Effect On Mice Breast Cancer Cell Line 4t1 After Knocking Down CXCR4/CXCR7

Objective: The breast cancer is one of the most common malignant tumors and seriously harm to women's physical and mental health and life.In the world,its incidence and mortality has has been ranked in the forefront of female malignant tumors.Conventional surgery,radiotherapy and chemotherty and cancer drug treatment can not solve the problem of recurrence and distant metastasis,which makes the molecular level study has attracted much attention,applied to breast cancer research,performance is also increasingly highlighted.Some studies have shown that CXCR4 and CXCR7,as receptors of the CXCL12,play a key role in regulating tumor growth,metastasis and spreading to other organs.At the same time,whether CXCR4 and CXCR7 can be used as a biomarker for tumor and as a potential target for new therapeutic method have become a hot research point and a challenge problem.In this study,we study the effect of CXCR4/CXCR7 on mice breast cancer cell lines after koncking-down CXCR4/CXCR7 expression.Method:1 4T1 cells were transfected with CXCR4-siRNA and CXCR7-siRNA and the biological characteristics of the transfected cell lines were observed under a microscope.2 Real time quantitative PCR and Western blotting were used to detect the expression of CXCR4 and CXCR7 in the stably transfected 4T1 cells,and the apoptosis of the cells was detected by flow cytometry.3 The invasion ability of 4T1 cells were detected by Transwell technique.4 VGEF and CyclinD1&Ki67 were detected by real-time quantitative PCR and western-blotting in stable transfected 4T1 cells.Results:1 After transfection with CXCR4-siRNA and CXCR7-siRNA,theexpression of CXCR4 and CXCR7 decreased significantly,and the morphology of 4T1 cells did not change,which could be further studied in cell biology.2 Knockdown of CXCR4 and CXCR7 expression could significantly increase the apoptosis of 4T1 cells.3 Knockdown of CXCR4,CXCR7 can inhibit cell proliferation in 4T1 cells,promote cell apoptosis and inhibit the invasion of cells.4 Knockdown of CXCR4 and CXCR7 can reduce the mRNA expression of VEGF,CyclinD1 and Ki67 in 4T1 cells..Conclusion:1 Low expression of CXCR4 and CXCR7 in mouse breast cancer cell line 4T1 can improve the apoptosis of tumor cells and inhibit the proliferation and invasion of tumor cells.2 low expression of CXCR4 and CXCR7 in mouse breast cancer cell line4T1 can reduce the expression of VEGF,CyclinD1 and Ki67.