Inhibition Effects Of Ginsenosides RG3 To Breast Cancer Cell Line

The incidence of cancer is increasing every year, and the desire to drugs of cancer is becoming much eager now. The ginsenoside Rg3 is the ingredient of ginseng, with previous reports of inhibition effect to cancer, We discuss the mechanism of Rg3 to the breast cancer cell line.From the following areas: (1)The effect of ginsenoside Rg3 on CXCR4. We use ginsenoside of Rh2 and Rg3 of 60μg/ml in the medium to culture with MDA-MB-231 cells for 24h in order to study the expression of the CXCR4 inhibition by immunocytochemistry, then we choose Rg3, the better one to the following experiments.While by the transwell assay,we use we use CXCL12 of 20nmol/ml, 100nmol/ml and 200nmol/ml as the inducer to test the chemotaxis activation of the cells cultured by Rg3 and it shows the best chemotaxis activation of Rg3 that while the concentration of CXCL12 is 100nmol/ml. (3) The role of flow cytometry over Rg3 on the MDA-MB-231 cells we identifie the G1 cell cycle increase 18.4% while S cell cycle decrease 15.0%, thereby we can sugget that it could make the cell cycle G1 blockade. (4)We detected the MAPK signaling pathway by immunocytochemistry. After the inhibition of 24h of 60μg/ml Rg3, the protein ERK's activation can be inhibited while After the inhibition of 6h of 60μg/ml Rg3 , the protein JNK's activation can be inhibited. The results shows that the ginenoside Rg3 could inhibit the expression of CXCR4,so to inhibit the metastastic ability,and otherwise, Rg3 can inhibit the MDA-MB-231 to G1 blockade,also inhibit the sub-pathway of ERK/MAPK and JNK/MAPK.