Effect Of SDF-1/CXCR4 On Proliferation And Apoptosis Of Breast Cancer Cell Line MCF-7

Objective: To explore the influence of SDF-1/CXCR4 biological axis onthe proliferation and apoptosis of tumor cells in the process of breast cancerformation, trying to reveal a new molecule system.Methods: We select breast cancer MCF-7 cell line as subject. In ourpresent research, through the Western blot,MTT,soft agar formation,flow cytometry, Hochest/PI and DNA ladder, we can observe the effect ofSDF-1/CXCR4 on the proliferation and apoptosis of MCF-7 cells after theaddition of the CXCR4 monoclonal antibodies (CXCR4 mAb).Results: 1,Western blotting analysis proved that breast cancer cellsMCF-7 express CXCR4 protein, and it is a membrane protein. 2,Thequalitative and quantitative detection of PCNA with MTT,soft agarformation and flow cytometry proved that the proliferation ability of MCF-7cells decreased obviously in vitro after the addition of CXCR4 mAb (P＜0.05,n=5), and the level of PCNA also decreased (78.1±3.6)％(n=3).3,After the treatment of the monoclonal antibodies (mAb) CXCR4 , theapoptosis of MCF-7 cells were measured with flow cytometry, Hochest/PIand DNA ladder. The expression of caspase-3 proteins were observed byWestern blotting analysis. The results showed the apoptotic rates increasedand the expression of caspase-3 protein increased relatively in MCF-7 cells. Conclusions: CXCR4 protein is a member of non-secreting protein. Theproliferation ability of MCF-7 cells decreased obviously in vitro after theaddition of CXCR4 mAb, while apoptosis increased. Thus SDF-1/CXCR4biological axis plays a crucial role in the formation of tumor by apoptosisand caspase-3 proteins may be one of the important transductions inapoptosis of MCF-7 cells. The experimental results revealed thatSDF-1/CXCR4 biological axis could mediate the crosstalk between tumorcells. These data afforded a new thinking and way for clinical therapy ofhuman breast cancer.