| Objective1.To observe the effect of vasoactive intestinal peptide(VIP)on the wound healing after corneal alkali burn in rats.2.To evaluate the use of vasoactive intestinal peptide on the expression of IL-1?and IL-6 in corneal alkali burn,and to explore the immunological mechanism after corneal alkali burn in rats.Methods1.Thirty-six healthy adult female Wistar rats were randomly divided into three groups: group A(normal blank control group,9),group B(corneal alkali burn saline control group,9),group C(corneal alkali burn 0.25g/L vasoactive intestinal peptide treatment group,9),group D(corneal alkali burn 0.50g/L vasoactive peptide treatment group,9).Formulating 0.25g/L and 0.50g/L vasoactive intestinal peptide eye drops.Group A did not deal with.Group B,C,D were produced moderate corneal alkali burn models.After alkali burn,group B,C and D were treated with 0.9% saline,0.25g/L vasoactive intestinal peptide and 0.50g/L vasoactive intestinal peptide respectively,5times a day.Every day after alkali burn,the anterior segment of the eyes were observed with a microscope and the corneal haze scores were scored.The corneal colored areas were measured by methylene blue staining on the 3rd,7th and 14 th day after alkali burn,and the healing rates of corneal epithelium were calculated.2.Forty-five healthy adult female Wistar rats were randomly divided into three groups: group A(normal blank control group,15),group B(corneal alkali burn saline control group,15)and group C(corneal alkali burn vasoactive intestinal peptide treatment group,15).Preparing 0.25g/L vasoactive intestinal peptide eye drops.Group A did not deal with.Group B and C were produced moderate corneal alkali burnmodels.After alkali burn,group B and C were treated with 0.9% saline and 0.25g/L vasoactive intestinal peptide respectively,5 times a day.Alkaloid burns were observed daily with anterior segment under the microscope.On the 3rd,7th and 21 st day,5 rats were randomly assigned to death in group A?B and C.The corneal tissues of alkali burn eyes were removed and divided into two equal parts.One part of HE staining was used to observe the changes of corneal tissue structure.The other part was used to detect the expression of interleukin-1?(IL-1?)and interleukin-6(IL-6)by corneal immunohistochemistry expression.Results1.On the 3rd day after alkali burn,there was no significant difference between the three groups in corneal opacity(P>0.05).On the 7th,14 th and 21 st day after alkali burn,the corneal turbidity of group C and group D was significantly lower than that of group B,the difference was statisticaly significant(P<0.05).There was no significant difference in corneal opacity between group B and group C(P>0.05).The average healing rates of corneal epithelium on the 3rd,7th and 14 th day after alkali burn were(35.61±4.88)%,(58.96±5.02)% and(68.44±2.52)% respectively in group B and were(42.88±5.82)%,(69.12±3.46)% and(80.60±5.14)% respectively in group C and were(42.23±5.18)%,(68.01±4.97)% and(80.54±5.84)% respectively in group D.On the 3rd,7th and 14 th day,the healing rates of corneal epithelium in group C and group D were significantly higher than those in group B,and the differences were statisticaly significant(P<0.05).There were no significant difference in the healing rates of corneal epithelium between group C and D at al time points(P>0.05).2.The mean optical densities of IL-1? detected by immunohistochemistry showed that on the 3rd day the average optical densities in group B and C were(1.80±0.20)×10-2 and(0.96±0.10)×10-2 respectively.On the 7th day,the group B and the group C were(1.66±0.15)×10-2and(0.74±0.11)×10-2respectively.On the 21 st day,the group B and the group C were(0.68±0.10)×10-2 and(0.25±0.13)×10-2 respectively.The average optical densities of IL-1? in rat corneas at each time point in group C were lower than those in group B,the differences were statisticaly significant(P<0.05).The mean optical densities of IL-6 detected by immunohistochemistry showed that the average optical densities in group B and C were(1.05±0.11)×10-2 and(0.57±0.10)×10-2respectively.On the 7th day,the group B and C were(1.33±0.13)×10-2and(0.88±0.12)×10-2respectively.On the 21 st day,the group B and C were(0.66±0.12)×10-2 and(0.37±0.12)×10-2.The mean optical densities of IL-6 in rat corneas at each time point in group C were significantly lower than those in group B(P <0.05).Conclusions1.Topical application of vasoactive intestinal peptide after corneal alkali burn in rats can reduce the corneal inflammatory response and promote the healing of corneas after alkali burn.2.Topical vasoactive intestinal peptide after corneal alkali burn can reduce the expression of IL-1? and IL-6 and reduce the infiltration of neutrophils and lymphocytes.VIP can reduce immune-inflammatory reaction caused by corneal damage in rats,and promote corneal healing. |
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